Folic acid derivatives and process for preparation

• US 4,314,988 A
• Filed: 10/31/1979
• Issued: 02/09/1982
• Est. Priority Date: 10/31/1979
• Status: Expired due to Term

• Current Assignee: Serono Diagnostics, Inc.
• Original Assignee: Baker Instruments Corporation
• Inventors: Farina, Peter R., Grattan, James A.
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/090,063
• Time in Patent Office: 832 Days
• Field of Search: 424/1, 424/12, 23/230 B, 544/257
• US Class Current: 436/505
• CPC Class Codes: G01N 33/82 involving vitamins or their receptors

Abstract

Folic acid derivatives, such as radiolabeled pteroyltyrosine, are conveniently synthesized from either pteroic acid or by the direct condensation of 6-formylpterin with p-aminobenzoyltyrosine methyl ester. The radioiodinated derivatives are particularly useful in competitive protein binding and radioimmuno-assays of folate compounds.

Claims

1. In a competitive protein binding assay of folic acid, its metabolites, or derivatives which comprises contacting a sample containing folic acid, its metabolites, or derivatives with a binder for folic acid, its metabolites, or derivatives and a radiolabeled acceptor composition capable of binding in competition with folic acid, its metabolites, or derivatives, incubating for a suitable time, separating and removing the unbound portions of the folic acid, its metabolities, or derivatives and counting the radioactivity of at least one of the said bound and unbound portions, said radiolabeled acceptor composition consisting of three linked components:

a substituted pteridine moiety, a p-aminobenzoyl moiety, and a radiolabel acceptor moiety, the improvement in said assay which consists of using as said radiolabel acceptor moiety an amino acid moiety or a des-carboxyamino acid moiety containing radioiodinated aromatic or heterocyclic ring attached to the p-aminobenzoyl moiety via an amide bond through a linear chain having no more than five atoms.

2. In the competitive protein binding assay of claim 1, the improvement in said assay wherein the radiolabel acceptor moiety in the said radioiodinated acceptor composition is a member of the group selected from ##STR8## wherein Z is selected from the group of hydrogen or lower alkyl.

3. In a radioimmunoassay of folic acid, its metabolites, or derivatives which comprises contacting a sample containing folic acid, its metabolites, or derivatives with an antibody for folic acid, its metabolites, or derivatives and a radiolabeled acceptor composition capable of binding in competition with folic acid, its metabolites, or derivatives, incubating for a suitable time, separating and removing the unbound portions of the folic acid, its metabolites, or derivatives and counting the radioactivity of at least one of the said bound and unbound portions, said radiolabeled acceptor composition consisting of three linked components:

a substituted pteridine moiety, a p-aminobenzoyl moiety, and a radiolabel acceptor moiety, the improvement in said assay which consists of using as said radiolabel acceptor moiety an amino acid moiety or a des-carboxyamino acid moiety containing radioiodinated aromatic or heterocyclic ring attached to the p-aminobenzoyl moiety via an amide bond through a linear chain having no more than five atoms.

4. In the radioimmunoassay of claim 3, the improvement in said radioimmunoassay wherein the radiolabel acceptor moiety in the said radioiodinated acceptor composition is a member of the group selected from ##STR9## wherein Z is selected from the group of hydrogen or lower alkyl.