Fermentation process

• US 4,316,956 A
• Filed: 02/06/1980
• Issued: 02/23/1982
• Est. Priority Date: 02/06/1980
• Status: Expired due to Term

• Current Assignee: NOVO Industri A/S Bagsvaerd
• Original Assignee: Novo Industri A/S (Novo Nordisk A/S)
• Inventors: Lutzen, Niels W.
• Primary Examiner(s): Penland, R. B.
• Application Number:
  US06/119,034
• Time in Patent Office: 748 Days
• Field of Search: 435/96, 435/42, 435/99, 435/161, 435/162, 435/813, 426/11, 426/13, 426/14, 426/29, 426/494
• US Class Current: 435/96
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

This invention relates to a novel fermentation process and in particular, to fermentative production of ethanol producing in the presence of non-gelled, or granular, starch particles, alpha-amylase and a glucoamylase; characteristic of the present process is recycle of enzymes for renewed use in fermentation, usually through termination of the fermentation prior to complete disappearance of the granular starch particles, and recovery for use anew of the unconsumed starch, along with enzymes thereon. Fermentation according to practice of this invention proceeds at a near to linear rate in the main.

Claims

1. A fermentation process which conists essentially of fermenting a 10%-45% w/w aqueous slurry of granular starch for the production of ethanol with an ethanol producing microorganism in the presence of alpha-amylase and gluco-amylase, the conduct of said fermentation being characterized by low levels of dextrin and fermentable sugars in solution in the fermentation broth throughout the fermentation, and, thereafter recovering enzymes from the fermentation broth for use anew in fermentation of granular starch.

2. The fermentation process of claim 1 wherein the process further comprises halting the fermentation prior to complete disappearance of granular starch, and thereafter recovering residual granular starch from the fermentation broth for later fermentation, enzymes being recovered on the residual starch.

3. The fermentation process of claim 1 carried out with a near to linear generation of CO₂ during the main course of the fermentation.

4. The fermentation process of claim 1 wherein the starch content of the slurry is in the range of 25-45% w/w and the granular starch and alcohol producing microorganism are removed from the fermentation broth for later fermentation, the enzymes being recycled with the granular starch.

5. The fermentation process of claim 1 wherein a thermostable alpha-amylase is employed and de-alcoholized fermentation broth is used anew in fermentation of granular starch, the de-alcoholized fermentation broth containing therein active alpha-amylase.

6. The fermentation process of claim 1 wherein the granular starch is pretreated with at least one of said enzymes at temperatures not exceeding the initial gelatinization temperature of starch prior to the fermentation.

7. A fermentation process which consists essentially of fermenting a 10%-45% w/w aqueous slurry of granular starch for the production of ethanol with an ethanol producing microorganism in the presence of alpha-amylase and gluco-amylase, the conduct of said fermentation being characterized by low levels of dextrin and fermentable sugars in solution in the fermentation broth throughout the fermentation, and a near to linear rate of CO₂ generation during the main course of the fermentation.

8. A fermentation process which consists essentially of fermenting a 10%-45% w/w aqueous slurry of granular starch for the production of ethanol at a temperature in the range of 25°

-38°

C. with an ethanol producing microorganism in the presence of fungal alpha-amylase in amounts within the range of 0.02-2.0 FAU/g of starch in the slurry or Bacillus amylase in the amount of 0.01-0.6 KNU/g of starch in the slurry and gluco-amylase in amounts within the range of 0.05-10.0 AGU/g of starch in the slurry, the conduct of said fermentation being characterized by low levels of dextrin and fermentable sugars in solution in the fermentation broth throughout the fermentation, and generation of CO₂ at a near to linear rate during the main course of the fermentation, and, thereafter recovering enzymes from the fermentation broth for use anew in fermentation of granular starch.