Glucose analyzer membrane containing immobilized glucose oxidase
First Claim
1. A composition for applying on a fluorocarbon treated to be hydrophillic to produce an immobilized glucose oxidase membrane for whole blood glucose analysis comprising:
- (a) a paraformaldehyde solution comprising about 4.0% paraformaldehyde in water;
(b) a bovine serum albumin solution comprising about 5% bovine serum albumin in water; and
(c) a glucose oxidase solution comprising about 10% glucose oxidase in water;
wherein each of the components (a), (b) and (c) are mixed together in a ratio of about 1;
1;
1.
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Abstract
A membrane and the method of making the same is disclosed for use with glucose analyzers to detect the level of glucose in liquid mediums such as whole blood. The membrane comprises a thin base wafer of a hydrophobic fluorocarbon material such as polytetrafluoroethylene (TEFLON). The wafer is initially etched by removing surface fluorides to produce a hydrophilic surface and is then contacted with a mixed reagent formed by combining in a volume ratio of about 1:1:1 about 4% paraformaldehyde in water, about 5% bovine serum albumin in water and about 10% glucose oxidase in water. The resultant membrane contains glucose oxidase covalently bonded to the surface of the base wafer.
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Citations
5 Claims
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1. A composition for applying on a fluorocarbon treated to be hydrophillic to produce an immobilized glucose oxidase membrane for whole blood glucose analysis comprising:
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(a) a paraformaldehyde solution comprising about 4.0% paraformaldehyde in water; (b) a bovine serum albumin solution comprising about 5% bovine serum albumin in water; and (c) a glucose oxidase solution comprising about 10% glucose oxidase in water; wherein each of the components (a), (b) and (c) are mixed together in a ratio of about 1;
1;
1.
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2. A membrane for use with a polarographic electrode for detecting the level of glucose in a liquid, said membrane comprising a base wafer of a fluorocarbon hydrophobic material, said wafer having bonded thereto a mixed reagent produced by combining (a) paraformaldehyde solution comprising about 4% paraformaldehyde in water, (b) a bovine serum albumin solution comprising about 5% bovine serum albumin in water, and (c) a glucose oxidase solution comprising about 10% glucose oxidase in water in the respective proportions of 1:
- 1;
1 by volume. - View Dependent Claims (3)
- 1;
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4. A method of producing a membrane for use with a polargraphic electrode for detecting the level of glucose in a liquid, said method comprising:
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(a) preparing a solution by combining about 4% paraformaldehyde in water, (b) preparing a solution by combining about 5% bovine serum albumin in water, (c) preparing a solution by combining about 10% glucose oxidase in water, (d) mixing the solutions of (a), (b) and (c) together in relative proportions of 1;
1;
1 by volume, and(e) preparing a base wafer of a fluorocarbon material and etching the surface thereof to remove surface fluorides, and (f) bonding said mixed solution of step (d) to the etched surface of the fluorocarbon material. - View Dependent Claims (5)
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Specification