Method and apparatus for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device

• US 4,318,480 A
• Filed: 08/20/1979
• Issued: 03/09/1982
• Est. Priority Date: 08/20/1979
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostic Incorporated
• Original Assignee: Ortho Diagnostics, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Hansen, W. Peter, Lombardo, Igino
• Primary Examiner(s): Rolla, Joseph J.
• Application Number:
  US06/068,113
• Time in Patent Office: 932 Days
• Field of Search: 209/3.1-3.3, 209/571, 209/579, 209/906, 209/127 R, 250/222 R, 250/222 PC, 356/39, 356/72, 356/73, 356/335, 356/338, 361/226, 364/413, 346/75
• US Class Current: 209/3.1
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1406 Control of droplet point
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

A novel method for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device is disclosed. The formation of the uniform droplets is carried out via the application of vibrational energy inparted by a transducer to a jetting laminar flow stream. Previously sensed particles contained within the core portion of the laminar stream are subsequently sorted from the stream as part of a subsequently formed droplet. The disclosed method uses the dependency of the droplet formation point on the amplitude of the wave form applied to the transducer and the modulation of this amplitude to control the droplet formation point distance. The position of the droplet formation point is detected via the use of a light source and photodetector focused on the jetting stream at the position at which the droplet formation point is to be located, and a method for automatically maintaining the breakpoint at that position is described.

Claims

1. An electrostatic particle sorting system, comprising:

(a) flow means for establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) particle sensing means for optically detecting said particles at least at said particle sensing point;

(c) perturbation means for perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream become a series of discrete droplets;

(d) droplet charging means for relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronization means for timing said relative charging such that said selected droplets contain at least selected particles detected by said optical detection means;

said synchronization means comprising;

(i) sheath sensing means for sensing at least the light scatter characteristic of said stream at a sheath sensing point therealong subsequent to said perturbation and for producing a surface character output signal which is proportional thereto;

(ii) surface character analysis means for producing an output signal which is at least selectively responsive to said surface character output signal; and

(iii) amplitude adjustment means responsive to said surface character analysis means for adjusting said amplitude of said perturbation means for establishing and maintaining said breakpoint at said sheath sensing point.

2. The invention of claim 1 wherein said amplitude adjustment means further comprises a transducer, transducer driver, and controlled amplitude oscillator means for acting through said transducer driver on said transducer to selectively vary said amplitude of perturbation applied to said stream.

3. The invention of claim 1 wherein said surface character analysis means comprises a wave form characteristics detector generator, reference wave form characteristics setting means for generating an output signal corresponding to desired wave form characteristics of the surface character output signal when the sheath sensing point is located at said breakpoint;

and an error signal generator for comparing the outputs of said reference wave form characteristic setting means and said wave form characteristic detector generator, said error signal generator comprising means for generating a proportional error signal in response to compared differences therebetween.

4. The invention of claim 1 wherein said sheath sensing means comprises a radiant energy source with respect to which the sheath stream portion is essentially translucent, and a radiant energy receptor for measuring at least the scatter of said radiant energy by said sheath stream portion and for producing an output signal proportional thereto.

5. The invention of claim 4 wherein said radiant energy source is a source of light energy.

6. The invention of claim 5 wherein said source of light energy is a helium-neon laser.

7. A method of electrostatically sorting particles comprising:

(a) establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) optically detecting said particles at least at said particle sensing point;

(c) perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronizing said relative charging such that said selected droplets contain at least selected particles detected in said optical detection step;

said synchronizing step comprising the steps of;

(i) sensing at least the light scatter characteristics of said stream at a sheath sensing point therealong downstream from said perturbing for producing a surface character output signal which is proportional thereto;

(ii) analyzing said surface character output signal to produce an output signal which is at least selectively responsive to said surface character output signal; and

(iii) adjusting said preselected amplitude in said perturbing step in response to said selectively responsive output signal to establish and maintain said breakpoint at said sheath sensing point.

8. The invention of claim 7 wherein said step of analyzing the surface character output signal comprises the steps of generating a current surface character output waveform, generating a reference waveform setting corresponding to a desired waveform characteristic of the surface character output signal when the sheath sensing point is located at said breakpoint, and comparing said waveforms to generate an error signal proportional to the compared differences therebetween.

9. The invention of claim 7 wherein said step of sensing the light scatter characteristics of said stream comprises the steps of selecting a radiant energy source with respect to which at least the sheath stream portion is essentially translucent, focusing said radiant energy source on at least said sheath stream portion at said sheath sensing point, and measuring at least the scatter of said radiant energy caused by said sheath stream portion at said sheath sensing point to produce said surface character output signal.

10. The invention of claim 9 wherein said step of selecting said radiant energy source comprises the step of selecting a light source with respect to which said sheath stream portion is essentially translucent.

11. The invention of claim 10 wherein said step of selecting said source of light energy comprises the step of selecting a helium-neon laser, the energy output of which is essentially translucent with respect to at least said sheath stream portion.

12. An electrostatic particle sorting system, comprising:

(a) flow means for establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) particle sensing means for detecting said particles at least at said particle sensing point;

(c) perturbation means for perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream become a series of discrete droplets;

(d) droplet charging means for relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronization means for timing said relative charging such that said selected droplets contain at least selected particles detected by said detection means;

said synchronization means comprising;

(i) sheath sensing means for sensing at least the light scatter characteristic of said stream at a sheath sensing point therealong subsequent to said perturbation and for producing a surface character output signal which is proportional thereto;

(ii) surface character analysis means for producing an output signal which is at least selectively responsive to said surface character output signal; and

(iii) amplitude adjustment means responsive to said surface character analysis means for adjusting said amplitude of said perturbation means for establishing and maintaing said breakpoint at said sheath sensing point.

13. A method of electrostatically sorting particles comprising:

(a) establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) detecting said particles at least at said particle sensing point;

(c) perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronizing said relative charging such that said selected droplets contain at least selected particles detected in said detection step;

said synchronizing step comprising the steps of;

(i) sensing at least the light scatter characteristics of said stream at a sheath sensing point therealong downstream from said perturbing for producing a surface character output signal which is proportional thereto;

(ii) analyzing said surface character output signal to produce an output signal which is at least selectively responsive to said surface character output signal; and

(iii) adjusting said preselected amplitude in said perturbing step in response to said selectively responsive output signal to establish and maintain said breakpoint at said sheath sensing point.