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Heterogenous specific binding assay employing a cycling reactant as label

  • US 4,318,980 A
  • Filed: 10/15/1979
  • Issued: 03/09/1982
  • Est. Priority Date: 04/10/1978
  • Status: Expired due to Term
First Claim
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1. In a heterogeneous specific binding assay method for determining a ligand in a liquid medium, which method comprises the steps of:

  • (a) contacting said liquid medium with reagent means including a labeled conjugate comprising a specific binding substance coupled to a labeling substance, said reagent means and the ligand forming a binding reaction system producing(1) a bound-phase of the labeled conjugate in which the specific binding substance therein is bound by a specific binding partner thereto and(2) a free-phase of the labeled conjugate in which the specific binding substance therein is not bound by a specific binding partner thereto,(b) separating said bound-phase and said free-phase; and

    (c) determining said labeling substance in said bound-phase or said free-phase as a function of the amount of said ligand in said liquid medium;

    the improvement wherein said labeling substance is a participant in a cyclic chemical reaction of the following type;

    ##STR57## wherein said labeling substance is a non catalytic material selected from the group consisting of reactants A, reactants B, and said cycled reactant.

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