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Fad-labeled specific binding assay monitored with apoglutathione reductase or apolipoamide dehydrogenase

  • US 4,318,983 A
  • Filed: 11/03/1980
  • Issued: 03/09/1982
  • Est. Priority Date: 06/04/1979
  • Status: Expired due to Term
First Claim
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1. A homogeneous specific binding assay method for determining a ligand in a liquid medium, comprising the steps of:

  • forming a reaction mixture by contacting said liquid medium with(a) reagent means including a labeled conjugate comprising, as label component, flavin adenine dinucleotide coupled to a binding component, such contact producing a binding reaction system in which a bound-species and a free-species of said labeled conjugate are formed, the proportion of the flavin adenine dinucleotide label component in said two formed species being a function of the presence of said ligand in said liquid medium, and(b) an apoenzyme selected from apoglutathione reductase and apolipoamide dehydrogenase, the ability of the flavin adenine dinucleotide label component to combine with the apoenzyme to produce a holoenzyme being different in activity in said two formed species, anddetermining the proportion of the flavin adenine dinucleotide label component in said two formed species by measuring holoenzyme activity in said reaction mixture.

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