Fractionation of protein mixtures by salt addition followed by dialysis treatment

• US 4,321,192 A
• Filed: 10/16/1980
• Issued: 03/23/1982
• Est. Priority Date: 01/10/1980
• Status: Expired due to Term

• Current Assignee: Hood Yacht Systems Corp., Ionics Incorporated (Veolia Environnement SA)
• Original Assignee: Ionics Incorporated (Veolia Environnement SA)
• Inventors: Jain, Surendar M.
• Primary Examiner(s): Prescott, Arthur C.
• Application Number:
  US06/197,441
• Time in Patent Office: 523 Days
• Field of Search: 204/180 R, 204/180 P, 260/112 R, 260/112 B, 260/121, 260/122, 424/12, 424/36, 424/177, 424/96, 424/101, 23/902, 23/913, 23/915, 23/230 B, 128/214 B, 128/DIG. 22, 210/646, 210/645, 210/647, 210/648
• US Class Current: 530/420
• CPC Class Codes: A23C 9/144 by electrical means, e.g. electrodialysis
• CPC Class Codes: A23J 1/00 Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
• CPC Class Codes: A23J 1/06 from blood
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61M 1/3482 by filtrating the filtrate using another cross-flow filter, e.g. a membrane filter
• CPC Class Codes: A61M 1/3486 Biological, chemical treatment, e.g. chemical precipitation; treatment by absorbents
• CPC Class Codes: B01D 61/44 Ion-selective electrodialysis
• CPC Class Codes: C07K 1/303 by salting out
• CPC Class Codes: C07K 1/36 by a combination of two or more processes of different types
• CPC Class Codes: C07K 14/765 Serum albumin, e.g. HSA
• CPC Class Codes: C07K 16/065 Purification, fragmentation
• CPC Class Codes: Y10S 128/22 Blood coagulation
• CPC Class Codes: Y10S 530/83 Plasma; serum

Abstract

A process is described for the fractionation of solutions of protein mixtures which includes the steps of subjecting such solutions to "salting-out", separation of the resulting turbidity, subsequent removal of the "salting-out" agent from the supernatant by dialysis and finally making up the original salt level of the liquid if required. Such a process may be used in therapeutic plasma exchange where removal of immunoglobulins or their complexes therefrom is desired so that the remaining albumin void of immunoglobulins can be administered back to the patient.

Claims

1. A process for separating an aqueous protein mixture into fractions having intrinsically distinguishable compositions comprising removing substantially all the turbidity therefrom, subsequently adding a salting-out agent thereto by passing said mixture into and out of a dialysis apparatus thereby increasing the ionic environment of the said mixture sufficiently to at least partially destabilize one or more proteins in said mixture, allowing said destabilized proteins to form turbidity, subsequently removing substantially all of said turbidity while maintaining the temperature of said mixture during the said separation in the range of between about 0°

-40°

C. and subsequently removing the salting-out agent by dialysis treatment.