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Fractionation of protein mixtures by salt addition followed by dialysis treatment

  • US 4,321,192 A
  • Filed: 10/16/1980
  • Issued: 03/23/1982
  • Est. Priority Date: 01/10/1980
  • Status: Expired due to Term
First Claim
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1. A process for separating an aqueous protein mixture into fractions having intrinsically distinguishable compositions comprising removing substantially all the turbidity therefrom, subsequently adding a salting-out agent thereto by passing said mixture into and out of a dialysis apparatus thereby increasing the ionic environment of the said mixture sufficiently to at least partially destabilize one or more proteins in said mixture, allowing said destabilized proteins to form turbidity, subsequently removing substantially all of said turbidity while maintaining the temperature of said mixture during the said separation in the range of between about 0°

  • -40°

    C. and subsequently removing the salting-out agent by dialysis treatment.

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