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Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same

  • US 4,324,858 A
  • Filed: 06/16/1980
  • Issued: 04/13/1982
  • Est. Priority Date: 06/16/1980
  • Status: Expired due to Term
First Claim
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1. A method for providing a treated porous material impregnated with a substantially stabilized cholinesterase comprising the steps of:

  • (a) uniformly distributing the cholinesterase through an aqueous medium buffered to a pH greater than about 6 but less than about 9 with a buffering amount of a zwitterionic buffering agent,(b) impregnating a porous material with the aqueous medium containing said cholinesterase, and(c) drying the resulting impregnated porous material to remove substantially all of the physically bound water and thereby provide said treated porous material, having substantially no measurable water vapor pressure in a closed system.

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