Method for detecting and controlling flow rates of the droplet forming stream of an electrostatic particle sorting apparatus

• US 4,325,483 A
• Filed: 08/20/1979
• Issued: 04/20/1982
• Est. Priority Date: 08/20/1979
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostics, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Original Assignee: Ortho Diagnostics, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Hansen, W. Peter, Barry, Donald E., Lombardo, Igino
• Primary Examiner(s): Rolla, Joseph J.
• Application Number:
  US06/068,231
• Time in Patent Office: 974 Days
• Field of Search: 209/3.1-3.3, 209/571, 209/579, 209/906, 209/127 R, 356/39, 356/72, 356/73, 356/335, 356/338, 250/222 R, 250/222 PC, 361/226, 364/413, 346/75
• US Class Current: 209/3.1
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1406 Control of droplet point
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

A novel method is disclosed for detecting and controlling the flow rate of a perturbed, droplet-forming stream in an electrostatic particle sorting apparatus. Detection apparatus is located at two points along the stream, at a first particle sensing point for sensing the presence of particles within a core portion of the stream, and at a second downstream point, preferably the stream breakpoint, for sensing light scatter and extinction characteristics which are proportional to the surface characteristics of the sheath portion of the stream. Changes in phase shift, pulse width, duty cycle, pulse area, or breakpoint location are detected by analyzing these sheath surface-related characteristics. An error signal is produced in response to such changes which drives an electromechanical fluid flow regulator to increase or decrease the fluid flow rate in a direction which tends to minimize the error signal. The flow rate is thus maintained at a reference flow rate setting.

Claims

1. An electrostatic particle sorting system, comprising:

(a) flow means for establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) optical detection means for optically detecting said particles at least at said particle sensing point;

(c) perturbation means for perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) droplet charging means for relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronization means for timing said relative charging such that said selected droplets contain at least selected particles detected by said optical detection means;

said synchronization means comprising;

(i) sheath sensing means for sensing at least the light scatter of said sheath stream portion at a sheath sensing point therealong subsequent to said perturbation and for producing a surface character output signal which is proportional thereto;

(ii) surface character analysis means for producing an output signal which is at least selectively responsive to said sheath sensing means;

(iii) flow rate adjustment means responsive to said surface character analysis means for establishing and maintaining a preselected flow rate of said stream.

2. The invention of claim 1 wherein said system further comprises a radiant energy source, said source providing energy for said optical detection means and for said sheath sensing means.

3. The invention of claim 1 wherein surface character analysis means comprises phase shift detection means for sensing shifts in phase of said surface character output signal.

4. The invention of claim 3 wherein said phase shift detection means comprises a phase reference generator, second means for generating a signal representative of actual phase conditions, and error signal generation means for comparing signals generated by said phase reference generator and said second means for generating to produce an error signal proportional to the differences therebetween.

5. The invention of claim 4 wherein said second means for generating comprises means for sensing at periodic intervals the value of at least a portion of said surface character output.

6. The invention of claim 5 wherein said second means for generating comprises interval establishment means for sensing said frequency of said perturbation means to thereby establish said intervals.

7. The invention of claim 1 wherein said surface character analysis means comprises breakpoint shift detection means for sensing shifts in the position of said breakpoint.

8. The invention of claim 7 wherein said breakpoint shift detection means comprises a breakpoint waveform generator, a second means for generating a signal representative of actual phase conditions, and an error signal generation means for comparing said breakpoint waveform and the other waveform from said second means to produce an error signal proportional to the direction of shift of said breakpoint from said sheath sensing point.

9. The invention of claim 8 wherein said second means comprises phase synchronization means for sensing said current waveform in a preselected phase.

10. The invention of claim 9 wherein said second means comprises means for sensing said frequency of said perturbation means to establish said phase.

11. The invention of claim 1 wherein said surface character analysis means comprises pulse width detection means for detecting the pulse width of said surface character output signal.

12. The invention of claim 11 wherein said pulse width detection means comprises a pulse width generator, a pulse width reference setting generator, and an error signal generator for comparing the outputs thereof and for producing an error signal in proportional response thereto.

13. The invention of claim 1 wherein said surface character analysis means further comprises a pulse area detection means for detecting the pulse area of said surface character output signal.

14. The invention of claim 13 wherein said pulse area detection means further comprises a pulse area generator, a pulse area reference setting generator, and an error signal generator means for comparing the output signals thereof and for producing a proportional error signal in response thereto.

15. The invention of claim 1 wherein said surface character analysis means further comprises a duty cycle detection means for detecting the duty cycle length of said surface character output signal.

16. The invention of claim 15 wherein said duty cycle detection means comprises a duty cycle generator, a duty cycle reference setting generator, and an error signal generator means for comparing the outputs thereof and for producing a proportional error signal in response thereto.

17. The invention of claim 1 wherein said sheath sensing means comprises a radiant energy source with respect to which the sheath stream portion is essentially translucent, and a radiant energy receptor for measuring at least the scatter of said radiant energy by said sheath stream portion and for producing an output signal proportional thereto.

18. The invention of claim 17 wherein said radiant energy source is a source of light energy.

19. The invention of claim 18 wherein said source of light energy is a helium neon laser.

20. The invention of claim 1 wherein said flow rate adjustment means comprises an electromechanical flow regulator driver, and an electromechanical proportional fluid flow regulator for at least altering the fluid flow of said sheath stream portion of said particle containing stream.

21. The invention of claim 20 wherein said flow rate adjustment means further comprises a differential pressure regulator for proportionally regulating the flow of said core stream portion with respect to variations in the flow of said sheath stream portion.

22. A method of electrostatically sorting particles comprising:

(a) establishing a flow of a continuous particle containing stream having at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) optically detecting said particles at least at said particle sensing point;

(c) perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) relatively charging said ones of said droplets as they are formed at said breakpoint; and

(e) synchronizing said relative charging such that said selected droplets contain at least selected particles detected by said optical detection step;

said synchronizing step comprising the steps of;

(i) sensing at least the light scatter characteristics of said sheath stream portion at a sheath sensing point therealong subsequent to the point of said perturbation for producing a surface character output signal which is proportional thereto;

(ii) analyzing said surface character output signal to produce an analyzed output signal which is at least selectively responsive to said sheath sensing means; and

(iii) adjusting the flow of at least a portion of said continuous particle containing stream at least in response to said analyzed output signal.

23. The invention of claim 22 wherein a single source of radiant energy is utilized as a radiant energy source for optically detecting said particles at least at said particle sensing point and as a radiant energy source for sensing the light scatter and extinction character of said sheath stream portion.

24. The invention of claim 22 wherein said step of adjusting said flow rate comprises the step of proportionally regulating the flow of said core stream portion with respect to said sheath stream portion.

25. The method of claim 22 wherein said step of analyzing said surface character output signal comprises the step of detecting shifts in phase in said surface character output signal.

26. The invention of claim 25 wherein the step of detecting the phase shift of said surface character output signal comprises the steps of producing a phase reference signal, generating a second signal representative of actual phase conditions, and comparing said reference signal and said second signal for producing an error signal proportional to the differences therebetween.

27. The invention of claim 26 wherein said step of producing said second signal comprises the step of sensing at periodic intervals the value of at least a portion of said surface character output signal.

28. The invention of claim 27 wherein said second signal detection step comprises the step of establishing said intervals by sensing said frequency caused by said perturbation step.

29. The invention of claim 22 wherein said surface character analysis step comprises the step of detecting shifts in the relative position of the breakpoint of said particle containing flow stream.

30. The invention of claim 29 wherein said step of detecting shifts in said breakpoint comprises the steps of generating a breakpoint waveform, generating a second signal representative of actual phase conditions, and comparing the breakpoint waveform and said second signal to produce an error signal proportional to the direction of shift of said breakpoint from said sheath sensing point.

31. The invention of claim 30 wherein said step of generating said second waveform signal comprises the step of synchronizing the phase of said waveform to produce said second signal in a preselected phase.

32. The invention of claim 31 wherein said step of generating said second waveform signal comprises the step of sensing the frequency of said perturbing to establish said preselected phase.

33. The invention of claim 22 wherein said step of analyzing said surface character comprises the steps of detecting the pulse width of said surface character output signal.

34. The invention of claim 33 wherein said step of detecting the pulse width comprises the steps of generating a pulse width signal, generating a pulse width reference setting signal, and comparing the outputs thereof for producing an error signal in proportional response thereto.

35. The invention of claim 22 wherein said step of analyzing the surface character further comprises the steps of detecting the pulse area of said surface character output signal.

36. The invention of claim 35 wherein said step of detecting said pulse area further comprises the steps of generating a pulse area signal, generating a pulse area reference setting signal, and comparing these said signals for producing a proportional error signal response thereto.

37. The invention of claim 22 wherein said step of analyzing said surface character further comprises the step of detecting the duty cycle length of said surface character output signal.

38. The invention of claim 37 wherein said step of detecting said duty cycle length comprises the steps of generating a duty cycle signal, generating a duty cycle reference setting signal, and comparing these said signals for producing a proportional error signal in response thereto.

39. The invention of claim 22 wherein said step of sensing said sheath comprises the steps of illuminating said sheath with a radiant energy source with respect to which the sheath stream is essentially translucent, and measuring at least the scatter of said radiant energy produced by said sheath stream portion to produce an output signal proportional thereto.

40. The invention of claim 39 wherein said step of illuminating said sheath stream with a radiant energy source comprises the step of illuminating said stream with a source of light energy.

41. The invention of claim 40 wherein said step of illuminating said sheath stream portion with light energy comprises the step of illuminating said sheath stream portion with a helium-neon laser.

42. an electrostatic particle sorting system, comprising:

(a) flow means for establishing the flow of a continuous particle containing stream comprising at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) detection means for detecting said particles at least at said particle sensing point;

(c) perturbation means for perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) droplet charging means for relatively charging selected ones of said droplets as they are formed at said breakpoint; and

(e) synchronization means for timing said relative charging such that said selected droplets contain at least selected particles detected by said detection means;

said synchronization means comprising;

(i) sheath sensing means for sensing at least the light scatter of said sheath stream portion at a sheath sensing point therealong subsequent to said perturbation and for producing a surface character output signal which is proportional thereto;

(ii) surface character analysis means for producing an output signal which is at least selectively responsive to said sheath sensing means;

(iii) flow rate adjustment means responsive to said surface character analysis means for establishing and maintaining a preselected flow rate of said stream.

43. A method of electrostatically sorting particles comprising:

(a) establishing a flow of a continuous particle containing stream having at least a particle containing core stream portion and a surrounding sheath stream portion, said stream having a particle sensing point defined therealong;

(b) detecting said particles at least at said particle sensing point;

(c) perturbing said stream with at least a preselected frequency and amplitude to cause said stream to form a breakpoint at which said stream becomes a series of discrete droplets;

(d) relatively charging said ones of said droplets as they are formed at said breakpoint; and

(e) synchronizing said relative charging such that said selected droplets contain at least selected particles detected by said detection step;

said synchronizing step comprising the steps of;

(i) sensing at least the light scatter characteristics of said sheath stream portion at a sheath sensing point therealong subsequent to the point of said perturbation for producing a surface character output signal which is proportional thereto;

(ii) analyzing said surface character output signal to produce an analyzed output signal which is at least selectively responsive to said sheath sensing means; and

(iii) adjusting the flow of at least a portion of said continuous particle containing stream at least in response to said analyzed output signal.