Deacetylated polysaccharide S-60

• US 4,326,052 A
• Filed: 08/14/1980
• Issued: 04/20/1982
• Est. Priority Date: 12/04/1978
• Status: Expired due to Term

• Current Assignee: Cp Kelco (JM Huber Corporation)
• Original Assignee: Merck & Co., Inc.
• Inventors: Colegrove, George T., Veeder, George T., Kang, Kenneth S.
• Primary Examiner(s): Brown, Johnnie R.
• Application Number:
  US06/178,053
• Time in Patent Office: 614 Days
• Field of Search: 536/1, 536/114, 260/112 R
• US Class Current: 536/123
• CPC Class Codes: A23G 2200/06 containing beet sugar or cane sugar if specifically mentioned or containing other carbohydrates, e.g. starches, gums, alcohol sugar, polysaccharides, dextrin or containing high or low amount of carbohydrate
• CPC Class Codes: A23G 3/346 Finished or semi-finished products in the form of powders, paste or liquids A23G3/343 takes precedence
• CPC Class Codes: A61L 9/048 air treating gels
• CPC Class Codes: C08B 37/006 Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 21/005 Glycopeptides, glycoproteins
• CPC Class Codes: C12R 2001/38 Pseudomonas
• CPC Class Codes: Y10S 516/02 Organic and inorganic agents containing, except water
• CPC Class Codes: Y10S 516/06 Protein or carboxylic compound containing
• CPC Class Codes: Y10S 530/825 Bacteria

Abstract

Deacetylated Polysaccharide S-60, prepared by deacetylating the polysaccharide S-60 produced by fermentation of Pseudomonas elodea, ATCC 31461, has valuable properties in both the clarified and non-clarified form, and is useful as an agar substitute or a shapeable room deodorant.

The non-clarified deacetylated gum contains about 17% protein and principally carbohydrate, which comprises glucuronic acid (˜13% based on wt. gum) and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:2.

The clarified, deacetylated gum contains mostly carbohydrate and no more than about 2% protein.

Claims

1. Deacetylated heteropolysaccharide S-60, which comprises (a) about 50% (wt./wt.) insoluble material of which about 34% (wt./wt.) is protein, and (b) about 50% (wt./wt.) carbohydrate which contains about 22-26% (wt./wt.) glucuronic acid, 0% acetyl groups, and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:

2, said rhamnose and glucose sugars being primarily 1,4 β

-linked, said heteropolysaccharide being further characterized in that it is anionic, and forms brittle, thermoreversible gels.

2. A process for preparing the compound of claim 1 which comprises heating a 1-5% aqueous solution of the heteropolysaccharide S-60 at a pH of about 10, at a temperature of 90°

-100°

C. for from 10 minutes to 45 minutes, and recovering the product thereby produced.

3. Deacetylated heteropolysaccharide S-60 produced by a process which comprises heating a 1-5% aqueous solution of heteropolysaccharide S-60 at a pH of about 10, at a temperature of 90°

-100°

C. for from 10 minutes to 45 minutes, and recovering the product thereby produced.

4. Deacetylated, clarified heteropolysaccharide S-60, which comprises no more than about 2% (wt./wt.) protein and carbohydrate said carbohydrate containing about 22-26% (wt./wt.) glucoronic acid, 0% acetyl groups, and the neutral sugars rhamnose and glucose in the approximate molar ratio 3:

2, said rhamnose and glucose sugars being primarily 1,4 β

-linked, said heteropolysaccharide being further characterized in that it is anionic, and forms brittle, thermoreversible gels.