Automated chemical testing apparatus

• US 4,328,185 A
• Filed: 06/26/1980
• Issued: 05/04/1982
• Est. Priority Date: 06/26/1980
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim Corporation (Roche Holding AG)
• Original Assignee: Boehringer Mannheim Corporation (Roche Holding AG)
• Inventors: Reasons, Robert, Albright, Bill E.
• Primary Examiner(s): Serwin, Ronald
• Application Number:
  US06/163,093
• Time in Patent Office: 677 Days
• Field of Search: 422/81, 422/82, 23/230 R
• US Class Current: 422/82
• CPC Class Codes: G01N 2035/0441 for samples
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G01N 35/1002 Reagent dispensers

Abstract

An automated chemical testing apparatus which includes a continuous flow front end portion and a discrete flow back end portion. Also provided is a collecting means for separately collecting these sample segments and the intersample segments, means for analyzing these sample segments, and a waste collection means for collecting waste fluids.

Claims

1. An automated chemical testing apparatus which comprises:

continuous flow means for providing a continuous flow stream of alternating sample and intersample fluid segments, each intersample fluid segment including a liquid wash segment;

collecting means for separately collecting each of the sample segments and for collecting the intersample segments including the wash segments separately of the sample segments;

analysis means for analyzing the sample segments;

waste collection means for collecting waste fluids said analysis means and said waste collection means each being connected with said collecting means; and

discrete flow means for alternately flowing a collected sample segment to said analysis means and an intersample segment to said waste collection means.

2. The apparatus of claim 1 in which said discrete flow means is for flowing the collected sample segments to said analysis means at a volumetric flow rate higher than the volumetric flow rate of the continuous flow stream.

3. The apparatus of claim 1 in which each of the sample segments is maintained stationary while the analysis means is analyzing the sample segment.

4. The apparatus of claim 2 in which each of the sample segments is maintained stationary while the analysis means is analyzing the sample segment.

5. The apparatus of claim 1 in which said discrete flow means includes a vacuum means for drawing the collected sample segments under vacuum into said analysis means.

6. The apparatus of claim 2 in which said discrete flow means includes a vacuum means for drawing the collected sample segments under vacuum into said analysis means.

7. The apparatus of claim 1 in which said continuous flow means provides intersample segments including a plurality of liquid wash segments separated by interwash air segments, and further provides a leading air segment adjacent the preceding sample segment and a following air segment adjacent the following sample segment.

8. The apparatus of claim 1 in which said collecting means comprises a receptacle connected to each of said analysis means and said waste collection means, said continuous flow means further being for delivering the continuous flow stream of alternating sample and intersample segments into the receptacle, said collecting means further comprising control means for directing the sample segments into said analysis means and for directing the intersample segments into the waste collection means.

9. The apparatus of claim 8 in which said continuous flow means provides intersample segments including a plurality of liquid wash segments separated by interwash air segments, and further provides a leading air segment adjacent the preceding sample segment and a following air segment adjacent the following sample segment.

10. The apparatus of claim 9 in which the leading and following air segments are each at least twice as large as any of the interwash air segments, said control means including bubble detecting means for detecting the leading and following air segments, said control means further being for directing the sample segments into said analysis means and for directing the intersample segments into the waste collection means in response to the detection of the leading and following air segments by the bubble detecting means.

11. The apparatus of claim 1 in which said continuous flow means includes flow path means for containing and transporting sample and intersample segments to said collecting means, sampling means for drawing sample and intersample segments into said flow path means, pump means for moving the sample and intersample segments through the flow path means, and reagent means for introducing reagent into the sample segments intermediate the sampling means and the collecting means.

12. The apparatus of claim 11 in which said flow path means includes an incubator through which the sample and intersample segments are transported intermediate of the sampling means and the collecting means.

13. The apparatus of claim 12 and further including diluent means for introducing diluent into the sample segments intermediate the sampling means and the collecting means.

14. The apparatus of claim 1 in which the sample segments as collected by said collecting means includes test components and waste components, said analysis means including a separator means for separating the test components from the waste components, said analysis means further including a measuring means for measuring an attribute of the test components of the sample segments, said discrete flow means further being for flowing the test components to the measuring means and for flowing the waste components to said waste collection means.

15. The apparatus of claim 14 in which said continuous flow means includes flow path means for containing and transporting sample and intersample segments to said collecting means, sampling means for drawing sample and intersample segments into said flow path means, pump means for moving the sample and intersample segments through the flow path means, and reagent means for introducing reagent into the sample segments intermediate the sampling means and the collecting means.

16. The apparatus of claim 15 in which said flow path means includes an incubator through which the sample and intersample segments are transported intermediate of the sampling means and the collecting means.

17. The apparatus of claim 16 and further including diluent means for introducing diluent into the sample segments intermediate the sampling means and the collecting means.