Novel polysaccharide and hypocholesterol composition containing the same

• US 4,330,533 A
• Filed: 04/24/1980
• Issued: 05/18/1982
• Est. Priority Date: 11/08/1977
• Status: Expired due to Term

• Current Assignee: Mitsubishi Kasei Corp. (Mitsubishi Chemical Group Corp.)
• Original Assignee: Mitsubishi Petrochemical Co., Ltd. (Mitsubishi Chemical Group Corp.), Mitsubishi YUKA Pharmaceutical Company Limited
• Inventors: Kanayama, Toshiji, Takayama, Yoshihiro, Mori, Motokuni, Masuda, Yoshiro, Endo, Fujio, Nozawa, Tsuneo
• Primary Examiner(s): BROWN, JOHNNIE
• Application Number:
  US06/143,442
• Time in Patent Office: 754 Days
• Field of Search: 424/181, 536/1, 435/101
• US Class Current: 514/54
• CPC Class Codes: A61K 31/715 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: A61P 3/06 Antihyperlipidemics
• CPC Class Codes: C12P 19/04 Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
• CPC Class Codes: Y10S 435/874 Pseudomonas

Abstract

New polysaccharide named M-30-C produced by cultivation of new strain Pseudomonas polysaccharogenes M-30 and having utility as hypocholesterol agents.

Claims

1. A polysaccharide having the following physicochemical properties:

(a) optical rotation;

[α

]_D²⁰ =+48.4°

±

3.3°

(0.1 w/v % aqueous solution);

(b) average molecular weight;

2.0×

10⁵ -2.0×

10⁶ ;

(c) elemental analysis;

C, 37.7%±

3.0%;

H, 5.8%±

0.5%;

(d) color reaction;

positive in anthrone reaction and phenolsulfuric acid reaction;

(e) determination of basicity, acidity or neutrality;

acidic polysaccharide;

(f) infrared absorption spectrum;

characteristic absorption bands (cm^-1) at 3450(S), 2940(M), 1620(M), 1400(M), 1040(S) and 890(W);

(g) solubility;

soluble in water, IN hydrochloric acid IN sulfuric acid and IN aqueous ammonia;

insoluble in ethanol, ether, acetone, chloroform and dimethyl sulfoxide;

(h) viscosity;

1000-3000 cps measured on its 1.0 w/v % aqueous solution at 20°

C. by means of BL-type viscometer (manufactured by Tokyo Keiki K. K., Japan);

(i) sugar units;

spots of glucose and mannose on paper chromatography after hydrolysis and sugar unit ratio of glucose to mannose of 3;

2 by gas chromatography; and

(j) color and shape;

white and amorphous as such and colorless in its aqueous solution.

2. A composition for reduction of cholesterol which comprises

(i) as an active ingredient an effective amount of a polysaccharide having the following physico-chemical properties:

(a) optical rotation;

[α

]_D²⁰ =+48.4°

±

3.3°

(0.1 w/v % aqueous solution);

(b) weight average molecular weight;

2.0×

10⁵ -2.0×

10⁶ ;

(c) elemental analysis;

C, 37.7%±

3.0%;

H, 5.8%±

0.5%;

(d) color reaction;

positive in anthrone reaction and phenol-sulfuric acid reaction;

(e) determination of basicity, acidity or neutrality;

acidic polysaccharide;

(f) infrared absorption spectrum;

characteristic absorption bands (cm^-1) at 3450(S), 2940(M), 1620(M), 1400(M), 1040(S) and 890(W);

(g) solubility;

soluble in water IN hydrochloric acid, IN sulfuric acid and IN aqueous ammonia;

insoluble in ethanol, ether, acetone, chloroform and dimethyl sulfoxide;

(h) viscosity;

1000-3000 cps measured on its 1.0 w/v % aqueous solution at 20°

C. by means of BL-type viscometer (manufactured by Tokyo Keiki K. K., Japan);

(i) sugar units;

spots of glucose and mannose on paper chromatography after hydrolysis and sugar unit ratio of glucose to mannose of 3;

2 by gas chromatography; and

(j) color and shape;

white and amorphous as such and colorless in its aqueous solution; and

(ii) a pharmaceutically acceptable carrier.