Dual isotope assays

• US 4,332,784 A
• Filed: 01/30/1980
• Issued: 06/01/1982
• Est. Priority Date: 02/06/1979
• Status: Expired due to Term

• Current Assignee: Amerlite Diagnostics Limited
• Original Assignee: The Radiochemical Centre Limited (General Electric Company)
• Inventors: Smith, Geoffrey F. W., Stevens, Ralph A. J., Jacoby, Benjamin
• Primary Examiner(s): Nucker, Christine M.
• Application Number:
  US06/116,776
• Time in Patent Office: 853 Days
• Field of Search: 424/1, 424/12, 23/230 B
• US Class Current: 436/500
• CPC Class Codes: G01N 33/78 Thyroid gland hormones , e.g. T3, T4, TBH, TBG or their receptors

Abstract

Dual isotope assays for thyroid function are performed by carrying out a radio-immunoassay for two of thyroxine (T4), tri-iodothyronine (T3), thyroid stimulating hormone (TSH), and thyroxine binding globulin (TBG) wherein a version of one of the thyroid components, preferably T4 or T3 is labelled with Selenium-75 and the version of the other thyroid component is labelled with a different radionuclide, preferably Iodine-125.

Claims

1. A method of testing for thyroid function by performing a radio-immunoassay of a sample by a dual isotope technique comprising the steps:

(a) mixing the sample to be assayed with a version of a first thyroid component selected from the group T3, T4, TSH and TGB labelled with Se-75 and a version of a second thyroid component selected from the said group labelled with another radioactive isotope, and with an antibody to the said first component and an antibody to the said second component, the amount of antibody being in each case insufficient for reaction with all the thyroid component and labelled version thereof that are present,

(b) incubating the mixture to effect reaction between the thyroid components and the labelled versions thereof, and their respective antibodies,

(c) separating the fractions of the thyroid components plus the labelled versions thereof that are bound to the antibodies from the fractions not so bound,

(d) measuring the radioactive concentrations of the bound or the unbound fractions, and

(e) using the measurements to calculate the concentrations of the thyroid components in the sample being assayed.

2. The method as claimed in claim 1, wherein the version of the other of the said thyroid components is labelled with I-125.

3. The method as claimed in claim 1, wherein T3 or T4 is labelled with Se-75, the version thereof having the formula:

##STR2## (a) R₁ is OH or a carboxyl protecting group

R₂ is -CO-CX₂ X₂ X₃

at least one of X₁, X₂ and X₃ is -A_p (SeQ)_q

A is saturated alkylene of 1 to 4 carbon atoms

Q is alkyl or alkenyl of 1 to 6 carbon atoms, cycloalkyl, aryl or aralkyl,

p is 0 or 1

q is 1 or 2

one of X₁, X₂ and X₃ may be amino or protected amino,

one or two of X₁, X₂ and X₃ may be H

M is H or I;

or

(b)

R₂ is H or an amino protecting group,

R₁ is -NZ-CY₁ Y₂ Y₃

at least one of Y₁, Y₂ and Y₃ is -A-(SeQ)_q

one of Y₁, Y₂ and Y₃ may be -COOH or protected carboxyl,

one or two of Y₁, Y₂ and Y₃ may be H

Z is H or -A-(SeQ)_q

A is saturated alkylene of 1 to 4 carbon atoms,

Q is alkyl or alkenyl of 1 to 6 carbon atoms, cycloalkyl, aryl or aralkyl,

q is 1 or 2

M is H or I.

4. The method as claimed in claim 3, wherein, in alternative (a), R₂ is selected from

CH₃.SeCH₂.CH(NH₂).CO--

CH₃.SeCH₂.CH(SeCH₃).CO--

CH₃.C(CH₂.SeCH₃).CO--

CH₃.SeCH₂.CH₂.CO--

C₆ H₅.CH₂.SeCH₂.CH₂.CO--.

5. The method as claimed in claim 3, wherein, in alternative (b), R₁ is selected from

CH₃.SeCH₂.CH₂.NH--

(CH₃.SeCH₂.CH₂)₂.N--

CH₃.C(CH₂.SeCH₃)₂.NH--

C₆ H₅.CH₂.SeCH₂.CH₂.NH--.

6. The method as claimed in claim 1, wherein the said first and second thyroid components are selected from the following combinations:

space="preserve" listing-type="tabular">______________________________________ FIRST COMPONENT SECOND COMPONENT ______________________________________ T3 T4 T4 T3 T4 TSH T4 TBG T3 TSH T3 TBG. ______________________________________

7. The method as claimed in claim 1, comprising the steps:

(a) mixing the sample to be assayed with a version of T4 labelled with Se-75 and a version of a selected member of the group T3, TSH and TBG labelled with I-125, and with an antibody to T4 and an antibody to the selected member of the group T3, TSH and TBG, the amount of antibody being in each case insufficient for reaction with all the thyroid component and labelled version thereof that are present,

(b) incubating the mixture to effect reaction between the thyroid components and the labelled versions thereof, and the antibodies,

(c) separating the fractions of the thyroid components plus the labelled versions thereof that are bound to the antibodies from the fractions not so bound,

(d) measuring the radioactive concentrations of the bound or the unbound fractions, and

(e) using the measurements to calculate the concentrations of the thyroid components in the sample being assayed.

8. The method as claimed in claim 7, wherein step (d) is performed by a single passage of the fraction through a dual chamber counter.

9. The method according to claim 7 in which at least one thyroid component is pre-incubated with its antibody before admixture with the labelled version of the said component.