Process for decomposing cells of biomasses or the like

• US 4,333,611 A
• Filed: 05/02/1980
• Issued: 06/08/1982
• Est. Priority Date: 05/05/1979
• Status: Expired due to Term

• Current Assignee: Dorr-Oliver Deutschland GmbH
• Original Assignee: Friedrich Josef Zucker
• Inventors: Osthaus, Georg, Zucker, Friedrich J., Zucker-Kerbler, Doris
• Primary Examiner(s): Meister, James M.
• Application Number:
  US06/145,915
• Time in Patent Office: 767 Days
• Field of Search: 241/1, 241/2, 241/23, 432/13
• US Class Current: 241/1
• CPC Class Codes: A23C 3/0335 the milk being heated by electrical or mechanical means, e.g. by friction
• CPC Class Codes: A23L 3/015 by treatment with pressure variation, shock, acceleration or shear stress or cavitation
• CPC Class Codes: A61L 2/02 using physical phenomena
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12N 1/066 by physical methods

Abstract

The decomposition of cells of biomasses or the like or of substrates containing biomass, e.g. for releasing cell content substances, for separating cell content and cell sheath or for inactivating enzymes or the like is performed by heating and expanding. According to the invention, the biomass or the like is heated by friction, optionally under pressure, to temperatures above the point of evaporation of water and it is subsequently expanded into atmosphere or in a reduced pressure. As for the apparatus, the cells of biomasses or the like are decomposed by means of a centrifugal machine in which a rotor and a stator with facing radial surfaces are coacting. Between the rotor and the stator, there is provided a gap producing frictional heat in a predetermined amount for the material passed continuously therethrough, and at the outlet of the gap, there is an expansion chamber which is in communication the atmosphere.

Claims

1. A process for decomposing cells of biomasses or the like or of substrates containing biomasses by heat and expansion, comprising passing said biomass through a gap between confronting surfaces of at least two elements, at least one of said elements moving with respect to the other of said elements at a sufficient speed and the width of said gap being sufficiently narrow to cause sudden heating of said biomass due to friction between said surfaces, and then directing said heated biomass from said gap into a chamber whereby the cells of said biomass are caused to burst due to the vapor generated in said cells by said heat.

2. The process of claim 1 wherein the cross-sectional width of said gap is between 2 μ

m and 200 μ

m.

3. The process of claim 2 wherein said confronting surfaces of said at least two elements are maintained at an acute angle with respect to each other.

4. The process of claim 2 or claim 3 wherein said elements having confronting surfaces defining said gap comprise a stator and a rotor respectively, the speed of said rotor being maintained between 500 rpm and 3000 rpm.

5. The process of claim 1 wherein said biomass is heated to at least a temperature at which water is caused to vaporize during its passage through said gap.

6. The process of claim 5 wherein a vacuum is maintained in said chamber to accelerate bursting of said vapor-filled cells.

7. The process of claim 1 wherein a continuous stream of said biomass flows through said gap and chamber.