Flow analyzer and system for analysis of fluids with particles

• US 4,338,024 A
• Filed: 05/02/1980
• Issued: 07/06/1982
• Est. Priority Date: 05/02/1980
• Status: Expired due to Term

• Current Assignee: IRIS International Inc. (Danaher Corporation)
• Original Assignee: IRIS International Inc. (Danaher Corporation)
• Inventors: DeForest, Sherman E., Bolz, Gunner
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US06/146,064
• Time in Patent Office: 795 Days
• Field of Search: 356/23, 356/39, 356/246, 250/461 B, 350/95, 235/92 PC, 340/146.3 CA
• US Class Current: 356/23
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/1484 microstructural devices
• CPC Class Codes: G01N 2015/1486 Counting the particles

Abstract

The method and apparatus for analyzing particles and particularly blood cells by conveying the particles along a broad shallow path where the path is magnified and converted into a series of still frame images with multiple particles in individual images. The still frame images are manipulated and combined as digital images to generate measures of the blood cells in the overall fluid sample giving different selected measures, such as, particle count, particle count of different kinds of cells, cell area, and cell count of different predetermined categories of cell type.

Claims

1. Apparatus for analysis of particles in a fluid which comprises

a flow chamber having an inlet and an outlet and shaped to convey fluid from the inlet to the outlet with particles in the fluid suspended in a path, a portion of said path having a width substantially greater than its thickness;

said path shaped to convey said fluid such that said particles are aligned in the flow stream substantially with their minimum cross-sectional area extended transverse to their direction of flow and their maximum cross-sectional area extended substantially parallel to said width;

microscopic means adapted to be focused on the path including an area of the path substantially greater than the area of the largest of the particles, and

image pick-up means for picking up substantially still frame images of particles in the path through the microscopic means.

2. The apparatus of claim 1 characterized further by the inclusion of processor means connected to the image pick-up means for analyzing the still frame images.

3. The apparatus of claim 2 characterized further by the inclusion of controller means coupled to the processor means for altering the condition of the fluid in response to analysis by the processor.

7. The apparatus of claim 1 or 4 in which said flow chamber comprises

a passageway extending from the inlet to the outlet with the passageway having an imaging area thereof on which said microscopic means is focused,

the passageway having a cross-sectional area which decreases substantially as the passageway extends from the inlet to the imaging area, a thickness which decreases substantially as the passageway moves from the inlet to the imaging area, and a width which decreases from the inlet and then increases substantially as the passageway moves to the imaging area.

4. Apparatus for analysis of predetermined particles in a liquid which comprises

a flow chamber having an inlet and an outlet and shaped to convey liquid from the inlet to the outlet with particles in the liquid suspended in a path, a portion of said path having a width at least ten times it thickness,

shear force distributing means for conveying said fluid such that said particles are aligned in the flow stream substantially with their minimum cross-sectional area extended transverse to their direction of flow and their maximum cross-sectional area extended substantially parallel to said width,

microscopic means adapted to be focused on the path including an area of the path at least one hundred times the area of the largest particles,

image pick-up means for picking up substantially still frame images of particles in the path through the microscopic means with the image pick-up means including means for storing at least one of the still frame images at a time in digital form, and

processor means connected to the image pick-up means for algebraically combining selected components of the recorded still frame images to generate algebraic sums representing the particles in multiple images.

5. The apparatus of claim 4 in which the flow chamber has supply means for supplying an envelope of fluid around the inlet and moving from the inlet to the outlet whereby the path for the particles is surrounded by the envelope of fluid.

6. The apparatus of claim 5 characterized further in that said apparatus contains a volume of liquid as said envelope of focusing fluid and sample cells as the predetermined particles.

8. The method of analyzing particles in a fluid which comprises

having a predetermined direction of motion while distributing the fluid sample over an extended area having a width and a thickness both measured perpendicular to the direction of flow, with the width many times the thickness, such that said particles are aligned in the direction of motion substantially with their minimum cross-sectional area extended transverse to the direction of motion and their maximum cross-sectional area extended substantially parallel to said width,

forming a series of still frame images of the fluid sample at a predetermined location in the flow path viewed in a direction parallel to the thickness, and

algebraically combining the number of particles located and their characteristics.

9. The method of claim 8 characterized further in that the fluid is a biological sample.

10. The method of claim 8 characterized further by the inclusion of the step of altering a condition of the fluid in response to the algebraic combination.

11. The method of claim 8 wherein said particles are asymmetrically shaped.

12. The method of claim 9 wherein said sample contains blood cells.

13. The method of claim 12 wherein said sample is blood.

14. The method of claim 12 wherein said sample is urine.

15. A flow chamber for analysis of fluid containing particles flowing therethrough said chamber comprising:

an inlet;

an outlet;

a path for said fluid flowing from said inlet to said outlet;

said path having a portion adapted for viewing with said portion having a width substantially greater than its thickness and an area substantially greater than the area of the largest of the particles;

said path having a cross-sectional area which decreases substantially as said path extends from the inlet to said portion, a thickness which decreases substantially as said path moves from the inlet to said portion, and a width which decreases from the inlet then increases substantially as said path moves to said portion.

16. The chamber of claim 15 wherein said fluid is a biological sample.