Stabilization of benzidine-type indicators with various enhancers

• US 4,339,242 A
• Filed: 07/23/1981
• Issued: 07/13/1982
• Est. Priority Date: 11/13/1979
• Status: Expired due to Fees

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Tabb, David L., Magers, Thomas A.
• Primary Examiner(s): Turk, Arnold
• Application Number:
  US06/285,929
• Time in Patent Office: 355 Days
• Field of Search: 252/408, 23/230 B, 23/901, 23/931, 23/932, 422/56, 435/14, 435/28
• US Class Current: 436/66
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 2326/12 3,3',5,5'-Tetramethylbenzidine, i.e. TMB
• CPC Class Codes: C12Q 2326/14 Ortho-Tolidine, i.e. 3,3'-dimethyl-(1,1'-biphenyl-4,4'-diamine)
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/153333 Halogen containing

Abstract

An improved composition, device and method, whereby enhanced sensitivity is achieved in detecting test sample constituents such as glucose and blood. The improvement resides in the use of a broad range of compounds as enhancers for benzidine-type indicators. The enhancer tends to stabilize the blue color form of such indicators.

Claims

1. In a composition for detecting the presence of a constituent in a test sample, said composition comprising a benzidine-type indicator, the improvement wherein said composition additionally comprises an enhancer compound having the structure ##STR5## wherein R is lower alkyl.

2. The composition of claim 1 in which said enhancer is 4-picoline.

3. A test device for determining the presence of a constituent in a test sample, said device comprising a carrier matrix incorporated with the composition of claim 1 or 2.

4. A method for determining the presence of a constituent in a test sample, said method comprising contacting said test sample with the improved composition of claim 1 or 2 and observing a detectable response.

5. A method for determining the presence of a constituent in a test sample, said method comprising contacting said test sample with the device of claim 3, and observing a detectable response.