Process for the preparation of long-chain dicarboxylic acids by fermentation
First Claim
1. A process for producing a long-chain dicarboxylic acid which comprises culturing a fungus belonging to Candida tropicalis capable of assimilating straight-chain hydrocarbons in a liquid medium containing a straight-chain hydrocarbon as a substrate to obtain a fermentation broth containing a long-chain dicarboxylic acid corresponding to said hydrocarbon, dissolving said dicarboxylic acid in the fermentation broth by adding an alkaline material to said broth, further adding diatomaceous earth to said fermentation broth, filtering it under pressure, precipitating the long-chain dicarboxylic acid in the thus obtained filtrate by heating said filtrate to a temperature above 50°
- C. while adjusting the pH value of the filtrate to 4 or below by adding a mineral acid to the filtrate, and then separating and collecting the precipitated long-chain dicarboxylic acid.
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Abstract
Disclosed in this invention is a process for producing a long-chain dicarboxylic acid by culturing a fungus belonging to Candida tropicalis which has the ability to produce a long-chain dicarboxylic acid in a liquid medium containing a straight-chain saturated hydrocarbon as substrate, the production of said dicarboxylic acid being phenomenally increased by properly adjusting the pH of the medium in the course of culture. There is also disclosed a method for advantageously separating and collecting said dicarboxylic acid from a fermentation broth containing said dicarboxylic acid produced by said culture.
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3 Claims
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1. A process for producing a long-chain dicarboxylic acid which comprises culturing a fungus belonging to Candida tropicalis capable of assimilating straight-chain hydrocarbons in a liquid medium containing a straight-chain hydrocarbon as a substrate to obtain a fermentation broth containing a long-chain dicarboxylic acid corresponding to said hydrocarbon, dissolving said dicarboxylic acid in the fermentation broth by adding an alkaline material to said broth, further adding diatomaceous earth to said fermentation broth, filtering it under pressure, precipitating the long-chain dicarboxylic acid in the thus obtained filtrate by heating said filtrate to a temperature above 50°
- C. while adjusting the pH value of the filtrate to 4 or below by adding a mineral acid to the filtrate, and then separating and collecting the precipitated long-chain dicarboxylic acid.
- View Dependent Claims (2, 3)
Specification