×

Potentiometric detection of hydrogen peroxide and apparatus therefor

  • US 4,340,448 A
  • Filed: 08/28/1978
  • Issued: 07/20/1982
  • Est. Priority Date: 08/28/1978
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for potentiometric detection of hydrogen peroxide comprisingproviding an electrolytic cell having a reference electrode and a working electrode composed at least in part of a hydrogen peroxide sensitive material,immobilizing at least one oxidase enzyme on said working electrode,said immobilized enzyme including catalase,introducing a hydrogen peroxide releasing substance into said cell,releasing said hydrogen peroxide by effecting contact between said enzyme and said hydrogen peroxide releasing substance,developing in said cell through interaction between said hydrogen peroxide and said working electrode an electrical potential which is a function of the concentration of said hydrogen peroxide in said electrolytic cell,maintaining the pH of said cell in the range of about 5 to 9, andmeasuring said electrical potential of said cell whereby said working electrode will serve as a support for said oxidase enzyme and also as a hydrogen peroxide sensitive electrode to provide an electrical potential signal which is a function of the concentration of said hydrogen peroxide.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×