Immunoadsorptive surface coating for solid-phase immunosubstrate and solid-phase immunosubstrate

• US 4,340,564 A
• Filed: 07/21/1980
• Issued: 07/20/1982
• Est. Priority Date: 07/21/1980
• Status: Expired due to Term

• Current Assignee: Whittaker Corp (Parker-Hannifin Corporation)
• Original Assignee: Daryl Laboratories, Inc. (Merck & Co., Inc.)
• Inventors: Harte, Richard A., Bart, Max
• Primary Examiner(s): Schor, Kenneth M.
• Application Number:
  US06/170,632
• Time in Patent Office: 729 Days
• Field of Search: 422/57, 422/56, 435/7, 435/177, 435/180, 435/5, 435/805, 424/8, 424/12, 424/1, 424/1.5, 260/29.6 R, 428/327, 428/411, 428/326, 428/325, 428/331, 428/461, 428/462, 428/442, 428/441, 428/521, 428/463, 428/522, 428/532, 428/264, 428/274, 252/408 R, 252/318, 23/915
• US Class Current: 422/426
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10T 428/252 Glass or ceramic [i.e., fired or glazed clay, cement, etc.] [porcelain, quartz, etc.]
• CPC Class Codes: Y10T 428/253 Cellulosic [e.g., wood, paper, cork, rayon, etc.]
• CPC Class Codes: Y10T 428/254 Polymeric or resinous material

Abstract

A solid-phase immunosubstrate (14) which has a surface coating (12) composed of a water based emulsion of microdiameter sized latex polymer beads (16) and materials which act as light scattering centers (17), said surface coating (12) having an immunoreagent added thereto to form an immunoreactive immunosubstrate (27). An overcoating (28) being formed on said surface coating (12), said overcoating having a higher percentage of similarly sized latex polymer beads (16) contained therein.

Claims

1. A solid-phase immunoreactive immunosubstrate for use in immunological assay determinations comprising:

a solid-phase surface;

a water based emulsion of dispersed latex polymer beads coating said surface and including latex polymer beads, water and an emulsifying agent to promote the emulsification of said beads within said water, said beads being of microdiameter size and composed of one of the substances from the group consisting of acrylic polymers, polyvinyl acetate polymers and butediene-styrene copolymers, and formed with a diameter of from 0.1 microns up to 1.0 microns, said coating further including a source of light scattering centers added to said coating to improve its fluorescent properties, whereby the concentration by weight of the components of said coating is approximately 15% light scattering centers, 15% polymer beads, 69% water and a 1% emulsifying agent;

an overcoating disposed over said coating and composed of a liquid emulsion having a composition by weight which includes 10-20% light scattering centers and 16-25% latex polymer beads of substantially identical size to those contained in said coating, plus a water vehicle in an amount sufficient to equal 100%, and having added to each 100 milliliters thereof, 0.5 grams of carboxymethyl cellulose, 1 milliliter of polyvinyl alcohol and 1 milliliter of hydroxypropylmethyl cellulose; and

an immunoreagent applied to at least one of said coating and said overcoating and adsorbed thereto to form an immunoreactive immunosubstrate for use in specific immunoreactions which require said immunoreagent.

2. A solid-phase immunoreactive immunosubstrate for use in immunological assay determinations comprising:

a solid-phase surface;

a water based emulsion of dispersed latex polymer beads coating said surface and including latex polymer beads, water and an emulsifying agent to promote the emulsification of said beads within said water, said beads being of microdiameter size and composed of one of the substances from the group consisting of acrylic polymers, polyvinyl acetate polymers and butediene-styrene copolymers, and formed with a diameter of from 0.1 microns up to 1.0 microns, said coating further including a source of light scattering centers added to said coating to improve its fluorescent properties;

said coating further including preservatives, surfactants, pH buffers, fungicides and defoamers, whereby the concentration by weight of the components of said coating is approximately 11% latex polymer beads, 11.3% light scattering centers, 0.5% preservatives, 0.1% pH buffers, 0.5% fungicides, 0.5% defoamers, 4.0% surfactants, 1.0% emulsifying agents, 0.01% immunoreagent and 71.09% water; and

an overcoating disposed over said coating and composed of a liquid emulsion having a composition by weight which includes 10-20% light scattering centers and 12-25% latex polymer beads of substantially identical size to those contained in said coating, plus a water vehicle in an amount sufficient to equal 100%, and having added to each 100 milliliters thereof, 0.5 grams of carboxymethyl cellulose, 1 milliliter of polyvinyl alcohol and 1 milliliter of hydroxypropylmethyl cellulose.