Apparatus and method for measuring the distribution of radiant energy produced in particle investigating systems

• US 4,341,471 A
• Filed: 01/02/1979
• Issued: 07/27/1982
• Est. Priority Date: 01/02/1979
• Status: Expired due to Term

• Current Assignee: Coulter Electronics, Inc.
• Original Assignee: Coulter Electronics, Inc.
• Inventors: Brunsting, Albert, Hogg, Walter R.
• Primary Examiner(s): Corbin, John K.
• Assistant Examiner(s): Koren, Matthew W.
• Application Number:
  US06/000,439
• Time in Patent Office: 1,302 Days
• Field of Search: 356/336, 356/338, 356/343, 250/574, 350/103, 350/109, 350/162, 350/211, 350/292, 350/320
• US Class Current: 356/343
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2021/4716 Using a ring of sensors, or a combination of diaphragm and sensors; Annular sensor
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence

Abstract

In an apparatus in which particles are passed through an optical sensing zone to measure their radiant energy distribution such as, for example, light scattering characteristics for the purpose of identifying the particles, means and a method are provided for deviating the collected light in accordance with predetermined different paths to a plurality of different photodetecting devices. The deviation is effected independently of collection by optical radiant energy transmitting or reflecting means. The different photodetecting devices enable the measurement of energy directed along the particular path which is identified with that device. The paths are established by the deviating means rather than permitted to evolve by the scattering phenomena themselves whereby the photodetecting devices can be located in convenient arrangements and may be conventional in construction. Different forms of the invention combine optical reflection or refraction for collecting the scattered radiant energy with optical reflection or refraction for deviation to achieve different advantages and results.

Claims

1. A method of measuring the directional distribution properties of the radiant energy of a particle for particle identification or the like which comprises:

A. passing the particle through a sensing zone and illuminating the particle with an incident beam of radiant energy,

B. collecting at least some of the directionally distributed radiant energy produced by the intersection of the particle and the beam and projecting the same toward a focal point in space remote from the sensing zone,

C. intercepting the projected portion of radiant energy and deviating the same along a plurality of different paths independently of the collecting,

i. each path being arranged to comprise the radiant energy gathered from a particular geometric area of the projected portion,

ii. the geometric areas being different,

iii. the axis of each path being deviated from extending toward said remote point in space,

iv. each path serving to cause confluence of the radiant energy of its said path at a particular location,

v. the locations of the respective confluences of radiant energy being spaced from one another, and

D. measuring the respective intensities of the radiant energy confluence at the respective locations where confluenced.

2. The method as claimed in claim 1 in which the radiant energy is scattered at the sensing zone.

3. The method as claimed in claim 2 in which primarily the back scattered radiant energy is collected.

4. The method as claimed in claim 2 in which both back scattered and forward scattered radiant energy are collected.

5. The method as claimed in claim 2 in which a portion of the scattered radiant energy is collected and projected by reflection.

6. The method as claimed in claim 5 in which the deviating is effected by reflection.

7. The method as claimed in claim 5 in which the deviating is effected by refraction.

8. The method as claimed in claim 7 in which the stream of liquid is enveloped in another stream of the same liquid to move through the sensing zone in sheath flow.

9. The method as claimed in claim 1 in which a portion of the scattered radiant energy is collected and projected by refraction.

10. The method as claimed in claim 9 in which the deviating is effected by reflection.

11. The method as claimed in claim 9 in which the deviating is effected by refraction.

12. The method as claimed in claim 11 in which the center of said arriving confluence of radiant energy is masked before measurement.

13. The method as claimed in claim 2 in which the focal point in space is chosen to be in alignment with the sensing zone and the axis of the incident beam, and the particle is passed through the sensing zone at a substantial angle relative to said beam axis.

14. The method as claimed in claim 2 in which the incident beam of radiant energy includes energy at a plurality of wave lengths, there being separate paths and locations for each respective wave length at different deviation angles from each geometric area.

15. The method as claimed in claim 2 in which the particle is entrained in a stream of liquid of a particular index of refraction and the sensing zone is immersed in a body of liquid having substantially the same index of refraction.

16. The method as claimed in claim 2 in which each confluence of radiant energy arriving at its respective location is shielded from all other radiant energy at said location to purify the same.

17. The method of making a fresnel element for use in measuring light scattering properties of particles which comprises:

A. forming a disc of segments of prisms, there being a plurality of concentric annuli of said segments, the annuli decreasing in radial dimension toward the axis of the disc,

B. at least each annulus of segments having its projected axis of deviation oriented differently from the orientation of the projected axes of deviation of the other annuli,

C. each of said axes of deviation being spaced from one another at least on a plane normal to the said axis of the disc and symmetrical about said disc at said plane,

D. using said disc as a master to make a mold and molding said fresnel element out of light transmitting material from said mold.

18. The method of making a fresnel element as claimed in claim 17 in which each segment has a projected axis of deviation different from the axes of projection of all other segments, the said axes of deviation of all segments meeting said plane form an area generally of the size of a television camera target capable of being scanned electronically.

19. Apparatus for measuring the directional distribution of radiant energy produced by particles for identification or the like of said particles which comprises:

A. a source of radiant energy arranged to project a beam of radiant energy along a first axis,

B. a sensing zone on said first axis,

C. means for moving particles through said sensing zone to produce scattering of the radiant energy from the beam,

D. means for collecting some of said scattered radiant energy and projecting same toward a focal point in space,

E. means for receiving the collected radiant energy before it reaches the focal point and deviating the same selectively with respect to different geometric portions of the collected energy to generate a plurality of different respective paths having different respective axes and confluence of radiant energy in said respective paths at different respective locations, and

F. means for measuring the intensity of the confluence of radiant energy at each location.

20. The apparatus as claimed in claim 19 in which the deviating means are reflective.

21. The apparatus as claimed in claim 19 in which the deviating means are refractive.

22. Apparatus as claimed in claim 19 in which the collecting means are radiant energy reflective.

23. Apparatus as claimed in claim 22 in which the collecting means comprise a generally ellipsoidal reflector.

24. Apparatus as claimed in claim 22 in which the collecting means comprise an ellipsoidal reflector having the sensing zone at its minor focus.

25. Apparatus as claimed in claim 24 in which said ellipsoidal reflector has a window on its axis adjacent its center, said deviating means being located thereat and comprising a refracting element.

26. Apparatus as claimed in claim 25 in which said ellipsoidal reflector has a second window on its axis at its end adjacent its minor focus and means on the exterior of the reflector to capture and refract distributed radiant energy emerging from said second window.

27. Apparatus as claimed in claim 25 in which the particle moving means include means for entraining said particles in a stream of first liquid, means are provided for filling the reflector with a second liquid having a refractive index similar to that of the first liquid and means are provided for leading at least most of the first liquid out of the reflector after it has passed through the sensing zone.

28. Apparatus as claimed in claim 27 in which means are provided to surround the stream of first liquid with an envelope of third liquid of a similar refractive index under pressure to cause the movement through the sensing zone to be sheath flow.

29. Apparatus as claimed in claim 19 in which the collecting means are radiant energy refractive.

30. Apparatus as claimed in claim 29 in which the collecting means comprise a lens system.

31. Apparatus as claimed in claim 19 in which the collecting means are both radiant energy refractive and radiant energy reflective.

32. Apparatus as claimed in claim 19 in which the particle moving means include means for entraining the particles in a liquid and means for flowing the liquid through the sensing zone.

33. Apparatus as claimed in claim 32 in which the collecting means comprise an ellipsoidal reflector, the sensing zone is at a focal point of the reflector, the reflector is enclosed and has a closure at an otherwise open end thereof which includes said deviating means, said reflector having means for filling the interior thereof with a second liquid of the same refractive index as that in which the particles are entrained and means are provided for leading the first liquid out of the reflector.

34. Apparatus for measuring the scattering of radiant energy produced by particles for identification or the like of said particles which comprises:

A. a source of radiant energy arranged to project a concentrated beam of said radiant energy along a first axis,

B. a sensing zone, said concentrated beam being directed to pass through said sensing zone,

C. means for moving particles in a stream of fluid to flow through said sensing zone at such an angle relative to said first axis and with such dilution in said fluid as to cause scattering of radiant energy by each particle as it passes through said sensing zone,

D. means for collecting at least some of said scattered radiant energy from certain scattering angles about said sensing zone and projecting same as a composite cone of varying intensity considered on a planar frontal aspect, the variations in intensity being related generally to the respective certain scattering angles, said composite cone being projected toward a focussing point in space on said first axis remote from said sensing zone,

E. radiant energy deviating means having a front surface and a rear surface interposed between said collecting means and said point in space and arranged to receive the projected radiant energy composite cone on its rear surface, transmit the same and have the radiant energy emerge from its front surface albeit in a different composition,

F. said radiant energy deviating means including a plurality of deviating components each constructed and arranged to deviate and focus and confluence the radiant energy of respective different geometric portions of the frontal aspect of said composite cone at locations forward of said front surface and lateral of said first axis whereby to produce a plurality of said locations, the locations being spaced apart and each being individual to a different geometric portion, and

G. photoresponsive means at said locations producing a separate signal for each location related respectively to the intensity of radiant energy confluenced at said locations.

35. The apparatus as claimed in claim 34 in which the collecting means are constructed to collect primarily back scattered radiant energy.

36. The apparatus as claimed in claim 34 in which the collecting means are constructed to collect both back scattered and forward scattered radiant energy.

37. The apparatus as claimed in claim 36 in which the annular components are constructed with optical dimensions derived from a common prism.

38. The apparatus as claimed in claim 37 in which the lens is a fresnel lens and the components are integral with one another.

39. The apparatus as claimed in claim 34 in which the collecting means are radiant energy transmissive in character.

40. The apparatus as claimed in claim 34 in which the collecting means are radiant energy reflective in character.

41. The apparatus as claimed in claim 40 in which the collecting means comprise an ellipsoidal reflector, the sensing zone being located at a focal point of said reflector.

42. The apparatus as claimed in claim 34 in which the collecting means include radiant energy reflection and transmission elements.

43. The apparatus as claimed in claim 34 in which the light deviating means comprise a lens and the deviating components are prismatic, each component being positioned to receive, transmit and deviate the radiant energy of a different one of said geometric portions, the angles of deviation being oriented at least circumferentially to space the resulting beam emerging from each component about said first axis to establish the spacing of said locations.

44. The apparatus as claimed in claim 43 in which all of said prismatic deviating components have the same angle of deviation.

45. The apparatus as claimed in claim 43 in which said lens is a fresnel lens and the components are integral with one another.

46. The apparatus as claimed in claim 43 in which the components are annular whereby the geometric portions are circular parts of said composite beam.

47. The apparatus as claimed in claim 43 in which the components include some which are semiannular and others of different geometric configuration.

48. The apparatus as claimed in claim 43 in which the lens is a fresnel lens and the components are integral with one another.

49. The apparatus as claimed in claim 34 in which the particle moving means include means for entraining the particles in a first liquid and means for flowing the first liquid through the sensing zone.

50. The apparatus as claimed in claim 49 in which the sensing zone is in a vessel and said vessel is adapted to be filled with a second liquid whose index of refraction is substantially the same as that of the first liquid.

51. The apparatus as claimed in claim 50 in which means are provided for surrounding the first liquid with a third liquid of refractive index similar to that of the first and second liquids under pressure to cause the movement of the first liquid through the sensing zone to be a sheath flow.

52. The apparatus as claimed in claim 51 in which the vessel has means for leading the first liquid out of the vessel to provide continuous flow thereof.

53. The apparatus as claimed in claim 50 in which the vessel is an ellipsoidal reflector and the collecting means comprise the interior surface of the reflector at least at one end thereof, the sensing zone being located at the focal point of that end, the reflector being open at its optical central portion with a radiant energy transmitting closure covering the opening and the said first mentioned transmitting means disposed at said opening.

54. The apparatus as claimed in claim 53 in which means are provided for leading the first liquid into the vessel on an angle with the optical axis of said ellipsoidal reflector, the said first axis being aligned with said optical axis of the ellipsoidal reflector and the first mentioned transmitting means are coaxial with said optical axis.

55. The apparatus as claimed in claim 54 in which said leading means include means for producing a sheath flow with a third liquid having the same index of refraction as the other liquids.

56. The apparatus as claimed in claim 34 in which said collecting means and transmitting means are located with respect to said sensing zone to collect and transmit primarily back scattered radiation, the said source being disposed on the same side of said sensing zone as said transmitting means.

57. The apparatus as claimed in claim 56 in which in addition to said first mentioned collecting and transmitting means, there are second collecting and transmitting means located with respect to said sensing zone to collect and transmit forward scattered radiation therefrom, deviate some to a second plurality of locations and second photoresponsive means at said second locations for measuring the intensity of radiant energy at said second locations.

58. The apparatus as claimed in claim 57 in which said collecting means is an ellipsoidal reflector with the sensing zone at a focal point of one end thereof, the first collecting means comprising the interior surface of said ellipsoidal reflector, the first transmitting means being spaced from said sensing zone away from said end and the ellipsoidal reflector being open thereat with the first transmitting means over the opening, there being an axial window at the said one end and the second collecting, transmitting, photoresponsive means and locations being outside of the reflector aligned with said window.

59. The apparatus as claimed in claim 56 which includes means for dumping the direct central radiant energy of said concentrated beam.

60. A composite mirror of multiple reflecting surfaces adapted to receive distributed radiant energy from a source that is directing such distributed radiant energy in a conical solid angle generally along an optical axis toward a focal point remote from said source, the mirror adapted to be disposed with its reflecting surfaces facing said source and arranged to reflect and deviate different geometric zones of the distributed radiant energy away from the focal point in different angles relative to said axis and to confluence thereof at respective separate locations where said confluenced portions of radiant energy from said respective zones can be measured.

61. The composite mirror of claim 60 in which the said surfaces are joined in a single structure.

62. The composite mirror of claim 60 wherein at most all of the reflecting surfaces are planar.

63. The composite mirror of claim 60 wherein each of the reflecting surfaces is planar and comprises a generally arcuate segment of a plane mirror tilted relative to said optical axis, at least some of the segments having radii different from one another whereby to deviate the radiant energy from said geometric zones to confluence at different locations.

64. The composite mirror as claimed in claim 63 wherein the arcuate segments are developments of the same planar mirror arranged at a tilt angle relative to said optical axis.

65. The composite mirror as claimed in claim 64 wherein each arcuate segment is a complete ellipsoidal ring and in addition to having a common angle of tilt is centered on a common point along said axis.

66. The composite mirror as claimed in claim 65 in which each segment has an angular disposition about said optical axis different from the others.

67. Apparatus for measuring the scattering of radiant energy produced by particles for identification or the like of said particles which comprises:

A. a source of radiant energy arranged to project a beam of radiant energy along a first axis,

B. a sensing zone on said first axis,

C. means for moving particles through said sensing zone to produce scattering of the radiant energy from the beam,

D. means for collecting some of said scattered radiant energy and projecting same toward a focal point in space and comprising an ellipsoidal reflector, said focal point lying on the optical axis of the reflector, the radiant energy being projected in the form of a conical solid angle whose apex would extend to said focal point,

E. means for receiving the collected radiant energy of said conical solid angle before it reaches said focal point and deviating the same selectively with respect to different geometric portions of the collected energy whereby to cause confluence of radiant energy at different locations, the deviation being a folding back of said projected radiant energy toward said reflector,

F. said locations being adjacent said reflector and

G. means for measuring the intensity of the confluence of radiant energy at each location.

68. The apparatus as claimed in claim 67 in which the means for receiving and deviating comprise a composite mirror of multiple reflecting surfaces adapted to receive distributed radiant energy from a source that is directing such distributed radiant energy in a conical solid angle generally along an optical axis toward a focal point remote from said source, the mirror adapted to be disposed with its reflecting surfaces facing said source and arranged to reflect and deviate different geometric zones of the distributed radiant energy away from the focal point in different angles relative to said axis and to confluence thereof at respective separate locations where said confluenced portions of radiant energy from said respective zones can be measured.

69. The apparatus as claimed in claim 68 in which each of the reflecting surfaces is planar and comprises a generally arcuate segment of a plane mirror tilted relative to said optical axis, at least some of the segments having radii different from one another in order to deviate the radiant energy from said geometric zones to confluence at different locations.

70. The apparatus as claimed in claim 69 in which the arcuate segments are developments of the same planar mirror arranged at a tilt angle relative to said optical axis and each arcuate segment is a complete ellipsoidal ring having a common angle of tilt and is centered on a common point along said optical axis such that each segment has an angular disposition about said optical axis different from the others.

71. A deviator element for use in an apparatus for measuring polar distribution radiant energy, said element being adapted for receiving a composite beam of distributed radiant energy on one surface, transmitting the radiant energy and deviating different portions of the composite beam in different directions and to different locations where the respective deviated beams can be measured, said element being formed of a plurality of prismatic components each having a geometric relation to a specific geometric portion of the composite beam adapted to be received and said components being joined in unitary form, wherein said element is generally circular, about half of the element being formed of semiannular portions of different radial dimension which are coaxial and the other half being formed of wedge shaped segments centered on the center of the circle.

72. A deviator element for use in an apparatus for measuring polar distribution radiant energy, said element being adapted for receiving a composite beam of distributed radiant energy on one surface, transmitting the radiant energy and deviating different portions of the composite beam in different directions and to different locations where the respective deviated beams can be measured, said element being formed of a plurality of prismatic components each having a geometric relation to a specific geometric portion of the composite beam adapted to be received and said components being joined in unitary form, wherein the respective components include a plurality of annuli of different radial dimension arranged coaxially, and wherein the angle of deviation of all components is the same but the axes of the deviated beams are pointed in different directions circumferentially spaced around the central element axis.

73. The element of claim 72 in which the prismatic components are fresnelled.

74. A deviator element for use in an apparatus for measuring polar distribution radiant energy, said element being adapted for receiving a composite beam of distributed radiant energy on one surface, transmitting the radiant energy and deviating different portions of the composite beam in different directions and to different locations where the respective deviated beams can be measured, said element being formed of a plurality of prismatic components each having a geometric relation to a specific geometric portion of the composite beam adapted to be received and said components being joined in unitary form, wherein the prismatic components are annuli of different radial dimension arranged coaxially with the radial width dimension of said respective annuli decreasing from the periphery toward the center.

75. The element of claim 74 in which all the components are dimensionally based upon the same optically dimensioned prism and have the same angle of deviation but are oriented to point the axes of the deviated beams emerging therefrom in different directions circumferentially spaced around the central element axis.

76. The element of claim 74 in which each annulus is formed from a plurality of different wedge-shaped segments arranged side by side circumferentially.

77. The element of claim 76 in which the ratio of radial width dimension to circumferential dimension of the majority of segments in about one to one.

78. The element of claim 76 in which the prismatic components are fresnelled.

79. A deviator element for use in an apparatus for measuring polar distribution radiant energy, said element being adapted for receiving a composite beam of distributed radiant energy on one surface, transmitting the radiant energy and deviating different portions of the composite beam in different directions and to different locations where the respective deviated beams can be measured, said element being formed of a plurality of prismatic components each having a geometric relation to a specific geometric portion to the composite beam adapted to be received and said components being joined in unitary form, wherein the prismatic components are annuli of different radial dimension arranged coaxially with the radial width dimension of said respective annuli decreasing from the periphery toward the center, all the components being dimensionally based upon the same angle of deviation but are oriented to point the axes of the deviated beams emerging therefrom in different directions circumferentially spaced around the central element axis, and wherein each annulus is formed of a plurality of different segments arranged side by side circumferentially.

80. The element of claim 79 in which the aspect ratio of a majority of segments is about one to one.

81. The element of claim 80 in which the prismatic components are fresnelled.

82. Method of making a deviator element for use in measuring radiant energy directional distribution properties of particles which comprises:

A. producing a prism of a given deviation angle,

B. cutting a plurality of mostly cylinders of graduated size from the prism,

C. assembling the cylinders telescopically and orienting each said cylinder by independent rotation thereof so its deviation direction is different from all others,

D. fixing the assembly to form a master; and

E. molding a deviator element from the master out of a radiant energy transmitting material.

83. The method as claimed in claim 82 in which the prism is made out of a radiant energy transmitting material and the orientation is effected by directing radiant energy through the rear of the assembly, rotating the cylinders independently and choosing the orientation of each cylinder by locating its radiant energy concentration location on a plane spaced from the front of the assembly.

84. The method as claimed in claim 82 or 83 wherein said prism is fresnelled.