Gravimetric diluter

• US 4,350,186 A
• Filed: 02/09/1981
• Issued: 09/21/1982
• Est. Priority Date: 02/09/1981
• Status: Expired due to Term

• Current Assignee: Spiral Biotech, Inc. (Advanced Instruments Inc.)
• Original Assignee: Spiral Systems, Inc.
• Inventors: Schalkowsky, Samuel, Whitley, Donald
• Primary Examiner(s): Schmidt, Frederick R.
• Application Number:
  US06/232,531
• Time in Patent Office: 589 Days
• Field of Search: 141/1, 141/83, 141/100, 141/102, 141/130, 141/192, 141/392, 177/70
• US Class Current: 141/83
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 2035/00217 involving measurement of weight

Abstract

An apparatus for making dilutions of a sample with a diluent to a predetermined dilution factor f by weight of the diluent is disclosed. A scale with TARE capability produces an output signal of the weight W of a sample within a container less the weight of the container. A dilution factor control is provided for setting the predetermined dilution factor f. Means are provided for calculating and storing the value TW=W/f which is the total weight of the sample plus the weight of the diluent which is required to dilute the sample to the predetermined dilution factor f. A pump is connected between a reservoir of diluent and the container for the sample for pumping the amount of diluent into the container which is required to dilute the sample to the predetermined weight dilution factor f. The scale provides a continuous output of the weight W of the sample plus diluent in the container at any instant in time. Means are provided for calculating when TW-W=0. In one embodiment, the flow of diluent to the container is stopped by the stopping of the pump when TW-W=0. In another embodiment, the flow of diluent to the container is stopped by closing a pair of valves located between the pump and the container when TW-W=0.

Claims

1. An apparatus for diluting a sample within a container to a dilution factor f by weight of diluent comprising:

(a) means for repeatedly measuring the weight W of the sample plus any diluent within the container;

(b) means for choosing a dilution factor f by weight of a diluent to be used for diluting the sample;

(c) means for calculating TW wherein TW=W/f and W is the weight of the sample within the container prior to the addition of diluent;

(d) means for calculating TW-W;

(e) fluid conducting means disposed between a reservoir of diluent and the container for permitting the diluent to flow from the reservoir to the container for the purpose of diluting the sample within the container to the selected dilution factor f; and

(f) means responsive to the means for calculating TW-W for controlling the flow of diluent between the reservoir and the container to cause diluent to flow between the container and the reservoir when TW>

W and to stop the flow of diluent when TW=W.

2. The apparatus of claim 1 wherein the means responsive to the means for calculating is a pump.

3. The apparatus of claim 2 wherein the pump is a peristalic pump.

4. The apparatus of claim 1 further comprising:

(a) means for calculating when TW-W=C where C is a predetermined weight and wherein

(b) the means for controlling maintains a higher flow rate when TW-W>

C and a lesser rate of flow when TW-W≦

C.

5. The apparatus of claim 4 wherein the means for controlling is a pump having two specified rates of flow, the pump pumping at the higher rate when TW-W>

C and at the lower rate when TW-W>

C.

6. The apparatus of claim 5 wherein the pump is a peristaltic pump.

7. An apparatus for diluting a sample within a container to a dilution factor f by weight of diluent comprising:

(a) means for repeatedly measuring the weight W of the sample plus any diluent within the container;

(b) means for choosing a dilution factor f by weight of a diluent to be used for diluting the sample;

(c) means for calculating TW wherein TW=W/f and W is the weight of the sample within the container prior to flow the addition of diluent;

(d) means for comparing TW and W;

(e) fluid conducting means disposed between a reservoir of diluent and the container for permitting the diluent to from the reservoir to the container for the purpose of diluting the sample within the container to the selected dilution factor f; and

(f) means responsive to the means for comparing TW and W for controlling the flow of diluent between the reservoir and the container to cause diluent to flow between the container and the reservoir when TW>

W and to stop the flow of diluent when TW=W.