Aminopyrine improved Trinder's reagent and dosing process for hydrogen peroxide from enzymatic oxidation of metabolic substrata with the same

• US 4,350,762 A
• Filed: 02/03/1981
• Issued: 09/21/1982
• Est. Priority Date: 02/04/1980
• Status: Expired due to Fees

• Current Assignee: ELVI SPA
• Original Assignee: ELVI SPA
• Inventors: De Luca, Ugo, Zoppi, Francesco
• Primary Examiner(s): Warden, Robert J.
• Application Number:
  US06/231,152
• Time in Patent Office: 595 Days
• Field of Search: 435/10, 435/11, 435/14, 435/15, 435/18, 435/19, 435/20, 435/25, 435/26, 435/28, 23/230 B, 23/909, 23/925, 252/408
• US Class Current: 435/10
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 1/60 involving cholesterol
• CPC Class Codes: C12Q 1/62 involving uric acid
• CPC Class Codes: C12Q 2326/00 Chromogens for determinations of oxidoreductase enzymes
• CPC Class Codes: C12Q 2326/96 4-Amino-antipyrine

Abstract

An aminopyrine-improved Trinder'"'"'s reagent is disclosed to be used for the dosing of hydrogen peroxide from enzymatic oxidation of metabolic substrata, such as glucose, cholesterol, uric acid, tryglycerides and coline. The use of such an improved reagent in Trinder'"'"'s reaction enables to avoid any interference from bilirubin, particularly in the determination of uric acid. The dosing process (for uric acid) is also disclosed by means of such a reagent.

Claims

1. In a Trinder'"'"'s reagent for the clinical analysis of hydrogen peroxide by the enzymatic oxidation of a metabolic substrate in the presence of bilirubin, said reagent containing 2,4-dichlorophenyl sulphonate and 4-aminophenazone;

the improvement in which the Trinder'"'"'s reagent contains 1 to 30 g/l of aminopyrine to block the interferring action of said bilirubin.

2. A reagent as claimed in claim 1, in which said aminopyrine is present in the amount of about 15 g/l.

3. A reagent as claimed in claim 1, containing peroxidase, about 2,000 I.U./l, 2,4-dichlorophenyl sulphonate-sodium salt about 1 g/l, monobasic potassium phosphate (KH₂ PO₄) about 18 g/l, sodium tetraborate (Na₂ B₄ O₇.10 H₂ O) about 13.9 g/l, 4-aminophenazone about 260 mg/l, aminopyrine about 15 g/l and uricase about 100 I.U./l.