Composition and test piece for measuring glucose concentration in body fluids

• US 4,353,984 A
• Filed: 09/02/1980
• Issued: 10/12/1982
• Est. Priority Date: 12/31/1978
• Status: Expired due to Term

• Current Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Original Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Inventors: Yamamoto, Takao, Yamada, Shigeki
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/205,367
• Time in Patent Office: 770 Days
• Field of Search: 435/14, 435/805, 252/408, 422/56, 23/901
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/203332 Hydroxyl containing

Abstract

A composition and test piece or dipstick for measuring the glucose concentration in body fluids such as urine, spinal fluid and blood. The composition includes a glucose oxidase, a peroxidase a N,N'"'"'-tetramethyldiaminophenylmethane and guaiac.

Claims

1. A diagnostic composition which comprises a glucose oxidase, a peroxidase, a N,N'"'"'-tetramethyldiaminophenylmethane and guaiac.

2. A composition as claimed in claim 1 wherein said N,N'"'"'-tetramethyldiaminophenylmethane comprises N,N'"'"'-tetramethyl 3,3'"'"'-diaminophenylmethane.

3. A composition as claimed in claim 1 wherein said N,N'"'"'-tetramethyldiaminophenylmethane comprises N,N'"'"'-tetramethyl-4,4'"'"'-diaminophenylmethane.

4. A composition as claimed in claim 3 wherein said guaiac and said N,N'"'"'-tetramethyl 4,4'"'"'-diaminophenylmethane are present in said composition in a weight ratio of from about 1:

1 to about 1;

10.

5. A composition as claimed in claim 4, wherein said guaiac and said N,N'"'"'-tetramethyl 4,4'"'"'-diaminophenylmethane are present in a weight ratio of from about 1:

2 to about 1;

4.

6. A composition as claimed in claim 5 further comprising a buffering agent.

7. A composition as claimed in claim 4 or 5 wherein said guaiac is selected from the group consisting of guaiac resin, guaiaconic acid, guaiarethene acid and guaiac acid.

8. A composition as claimed in any of claims 3-5 further comprising polyvinylbutyral.

9. A test piece comprising a substrate supporting a diagnostic composition which comprises a glucose oxidase, a peroxidase, guaiac and a N,N'"'"'-tetramethyldiaminophenylmethane.

10. A test piece as claimed in claim 9 wherein said N,N'"'"'-tetramethyldiaminophenylmethane comprises N,N'"'"'-tetramethyl-4,4'"'"'-diaminophenylmethane.

11. A test piece as claimed in claim 10 further comprising polyvinylbutyral.