Molecule selective enzyme electrode

• US 4,354,913 A
• Filed: 12/19/1980
• Issued: 10/19/1982
• Est. Priority Date: 06/21/1978
• Status: Expired due to Fees

• Current Assignee: Radelkis Elektrokemiai Muszergyarto Szovetkezet
• Original Assignee: Radelkis Elektrokemiai Muszergyarto Szovetkezet
• Inventors: Pungor, Erno, Nagy, Geza, Havas, Jeno, Porjesz, Emma
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/218,104
• Time in Patent Office: 669 Days
• Field of Search: 204/195 B, 204/195 P, 204/1 E, 435/817, 435/288, 435/175, 435/177
• US Class Current: 204/403.08
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00316 Detecting door closure
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A molecule selective sensor has an enzymatically active membrane which is prepared by reacting an enzyme with glutaric aldehyde on the surface of a protein membrane found in nature. Exemplary is the reaction of glucoseoxidase with glutaric aldehyde on the surface of hog intestine membrane.

Claims

1. A hollow molecule-selective sensor having a sensing electrode, a reference electrode and a permeable, enzymatically active membrane comprising a self-supporting, 10-15 micron thick protein membrane found in nature which closes the bottom of the sensor, said membrane containing on its surface the reaction product solely of an enzyme and glutaric aldehyde.

2. A sensor as in claim 6, wherein the said protein membrane is selected from the group consisting of hog intestine membrane, ewe intestine membrane, air bladder of fish, epidermal section of a mammal, epidermal section of a reptile and corneal section.