Amino-functionalized phthalhydrazide intermediates

• US 4,355,165 A
• Filed: 03/20/1980
• Issued: 10/19/1982
• Est. Priority Date: 07/24/1978
• Status: Expired due to Term

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Carrico, Robert J., Boguslaski, Robert C., Christner, James E.
• Primary Examiner(s): RIZZO, NICHOLAS
• Application Number:
  US06/131,881
• Time in Patent Office: 943 Days
• Field of Search: 544/237, 544/235
• US Class Current: 544/237
• CPC Class Codes: C07D 209/48 with oxygen atoms in positions 1 and 3, e.g. phthalimide
• CPC Class Codes: C07D 237/32 with oxygen atoms directly attached to carbon atoms of the nitrogen-containing ring
• CPC Class Codes: G01N 33/533 with fluorescent label

Abstract

Amino-functionalized phthalhydrazide intermediates of the formula: ##STR1## wherein one of R⁵ and R⁶ is hydrogen and the other is --NR⁷ R⁸ ; R⁷ is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R⁸ is ##STR2## wherein n=1-3. The compounds are intermediates in the synthesis of chemiluminescent-labeled conjugates which are useful as reagents in specific binding assays for determining ligands or their specific binding partners in liquid media.

Claims

1. A compound of the formula:

##STR49## wherein one of R⁵ and R⁶ is hydrogen and the other is --NR⁷ R⁸ ;

R⁷ is hydrogen or straight chain alkyl containing 1-4 carbon atoms and R⁸ is ##STR50## wherein n=1-3.

2. The compound of claim 1 wherein R⁵ is --NR⁷ R⁸.

3. The compound of claim 2 wherein n=1.

4. The compound of claim 3 wherein R⁷ is hydrogen.

5. The compound of claim 3 wherein R⁷ is ethyl.