Method of treating collected mammal semen and separating sperm into X Y components
First Claim
1. A method of preserving semen for use in artificial insemination comprising the steps of:
- collecting semen from a mammal;
treating the mammal semen by adding mammal saliva to prevent coagulation;
diluting the semen with a diluting solution; and
storing the semen in a water bath at substantially the body temperature of the mammal.
1 Assignment
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Accused Products
Abstract
A method of treating mammal semen for use in artificial insemination is provided. Human saliva is added to the semen as an anti-coagulant. For mammals other than primates and voles, sperm-free human semen is added to the mammal semen as a cell membrane diffusion material so that a dye such as quinicrine or quinicrine mustard will be absorbed by the Y chromosome. The semen is then fed in a narrow stream through a detection chamber where it is irradiated with ultraviolet light causing the Y chromosomes to fluoresce. The stream is fed from the detection chamber through a fluidic amplifier wherein the stream is switched in response to whether the detected chromosome is fluorescent or non-fluorescent so that the fluorescent Y chromosome is directed through one outlet port and collected and the non-fluorescent X chromosome is directed through a second outlet port and collected.
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Citations
13 Claims
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1. A method of preserving semen for use in artificial insemination comprising the steps of:
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collecting semen from a mammal; treating the mammal semen by adding mammal saliva to prevent coagulation; diluting the semen with a diluting solution; and storing the semen in a water bath at substantially the body temperature of the mammal. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of separating X and Y chromosomes in mammal semen comprising the steps of:
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collecting semen from the mammal; adding about 1.0 ml of mammal saliva to about 2.0 ml to about 3.5 ml of mammal semen to prevent coagulation; diluting the semen on a 1;
1 ratio with a diluting solution;maintaining the temperature of the treated and diluted semen at a temperature of about 37°
C.;adding a cell membrane diffusion material to the semen so that a dye can penetrate the cell membrane of the sperm containing the Y chromosome; adding a dye to the semen to cause only the Y chromosome to fluoresce under ultraviolet light; irradiating the semen with ultraviolet light; moving the semen in a stream through a detection chamber; sensing the presence of either a fluorescent Y chromosome or a non-fluorescent X chromosome in the detection chamber; providing an output signal in response to the sensed chromosome which varies according to whether the chromosome is fluorescent or non-fluorescent; passing the stream from the detection chamber through a fluidic amplifier; and switching the stream in response to each sensed chromosome to one of two outlet ports so that the fluorescent Y chromosomes are directed through one outlet port and collected and the non-fluorescent X chromosomes are directed through the other outlet port and collected. - View Dependent Claims (8, 9, 10, 11)
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12. A method of separating sperm containing X and Y chromosomes from semen which has been dyed to cause penetration of the dye only into the Y chromosome, comprising the steps of:
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moving the semen in a stream through a detection chamber; irradiating the semen with ultraviolet light; sensing the presence of either a fluorescent or non-fluorescent chromosome in the stream in the detection chamber; providing an output signal in response to the sensed chromosomes which varies according to whether the chromosome is fluorescent or non-fluorescent; passing the stream from the detection chamber through a fluidic amplifier; and switching the stream in response to each sensed chromosome in the detection chamber to direct the sensed chromosome to one of two outlet ports so that the fluorescent Y chromosomes are directed through one outlet port and collected and the non-fluorescent X chromosomes are directed through the other outlet port and collected. - View Dependent Claims (13)
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Specification