Stabilization of glucose oxidase apoenzyme

• US 4,366,243 A
• Filed: 04/17/1981
• Issued: 12/28/1982
• Est. Priority Date: 04/17/1981
• Status: Expired due to Fees

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Rupchock, Patricia A., Tyhach, Richard J.
• Primary Examiner(s): Kepplinger, Esther M.
• Application Number:
  US06/255,310
• Time in Patent Office: 620 Days
• Field of Search: 435/25, 435/28, 435/7, 435/188, 435/190, 435/805, 435/810, 435/13, 435/18, 435/21, 435/26, 435/20, 435/22, 435/19, 435/16, 230/230 B
• US Class Current: 435/7.7
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/963 Methods of stopping an enzyme reaction or stabilizing the test materials
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 435/975 Kit

Abstract

A stabilized composition comprising apoglucose oxidase is disclosed. In addition to the apoenzyme, a stabilizing agent comprising poly(vinyl alcohol), bovine serum albumin or mixtures thereof is included. The composition can also comprise, in addition to the apoenzyme and stabilizing agent, reagents for a homogeneous specific binding assay system capable of producing a detectable response which is a function of a ligand in, or the ligand binding capacity of a test sample, wherein the apoenzyme is itself one of the reagents in the assay system. The stabilized composition can be utilized in a test device for assaying a ligand in, or the ligand binding capacity of a liquid sample, whereby the device comprises a test device incorporated with the composition.

Claims

1. A stabilized glucose oxidase apoenzyme composition comprising glucose oxidase apoenzyme and a stabilizing agent comprising poly(vinyl alcohol) and bovine serum albumin.

2. The composition of claim 1 in which said composition is in the dry state.

3. A composition for determining the presence of a ligand in, or the ligand binding capacity of, a liquid test sample, comprising reagents for a homogeneous specific binding assay system capable of producing a detectable response which is a function of the presence of said ligand or said ligand binding capacity, wherein one of said reagents is glucose oxidase apoenzyme, and a stabilizing agent comprising poly(vinyl alcohol) and bovine serum albumin.

4. The composition of claim 3 in which said reagents are in the dry state.

5. A test device for determining the presence of a ligand in, or the ligand binding capacity of, a liquid test sample, comprising a carrier matrix incorporated with the composition of claim 3 or 4.