Process for enriching the protein content of food and feedstuff and products thereof
First Claim
1. A process for increasing the protein content and improving the overall amino acid balance of a cereal grain which contains carbohydrates, without substantially altering the protein present in the cereal grain, by fixing nitrogen from a gaseous mixture containing molecular nitrogen comprising the steps of:
- (a) forming a mixture of from about 40% to about 60% by weight cereal grain and from about 40% to about 60% by weight water;
(b) sterilizing said mixture in order to substantially eliminate the growth of substances which might compete with the growth of a spawn culture of the genus Pleurotus;
(c) inoculating said mixture with from about 1% to about 5% by weight of at least one member selected from the group consisting of Pleurotus ostreatus, Pleurotus ulmarius, Pleurotus sapidus, Pleurotus cornucopiae, and Pleurotus florida;
(d) maintaining the inoculated mixture in the presence of a mixture of a gaseous mixture of molecular nitrogen and oxygen at a temperature of from about 10°
C. to about 46°
C. for from about 21 to about 35 days so as to enable the mycelium of said spawn culture of the genus Pleurotus to reproduce; and
(e) recovering from the inoculated mixture as a food product the cereal grain with an increased protein content and improved amino acid balance without substantial alteration of the protein initially present.
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Abstract
The invention comprises a method for producing a protein enriched food or feed product and products thereof. The method entails forming a mixture of water and a food or feed product, sterilizing the mixture, innoculating the sterilized mixture with a spawn culture of the genus Pleurotus, maintaining the innoculated mixture in the presence of air at a temperature of from about 5° to about 46° C. so as to enable the mycelium of the spawn culture to grow, and later terminating the growth of the mycelium. The food or feed product resulting from this process has an increased protein content.
30 Citations
2 Claims
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1. A process for increasing the protein content and improving the overall amino acid balance of a cereal grain which contains carbohydrates, without substantially altering the protein present in the cereal grain, by fixing nitrogen from a gaseous mixture containing molecular nitrogen comprising the steps of:
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(a) forming a mixture of from about 40% to about 60% by weight cereal grain and from about 40% to about 60% by weight water; (b) sterilizing said mixture in order to substantially eliminate the growth of substances which might compete with the growth of a spawn culture of the genus Pleurotus; (c) inoculating said mixture with from about 1% to about 5% by weight of at least one member selected from the group consisting of Pleurotus ostreatus, Pleurotus ulmarius, Pleurotus sapidus, Pleurotus cornucopiae, and Pleurotus florida; (d) maintaining the inoculated mixture in the presence of a mixture of a gaseous mixture of molecular nitrogen and oxygen at a temperature of from about 10°
C. to about 46°
C. for from about 21 to about 35 days so as to enable the mycelium of said spawn culture of the genus Pleurotus to reproduce; and(e) recovering from the inoculated mixture as a food product the cereal grain with an increased protein content and improved amino acid balance without substantial alteration of the protein initially present. - View Dependent Claims (2)
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Specification