Immobilization of biological material with an acrylonitrile polymer
First Claim
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1. An immobilized material comprising a biologically active enzyme bound by covalent bonding with a crosslinking agent or condensing agent onto a carrier which is a water-insoluble acrylonitrile polymer containing from 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer and having a porous structure containing a number of micro-pores with an average size of from 40 to 9000 A, said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride.
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Abstract
Biological material such as enzymes is immobilized by adsorption or covalent bonding to a micro-porous water-insoluble acrylonitrile polymer containing from 20 μM to 1000 μM of amino groups per gram of the polymer. Covalent bonding may be carried out with a crosslinking or condensing agent. The amino groups may be introduced into the polymer by reduction of nitrile groups with lithium aluminum hydride in an inert non-solvent.
53 Citations
71 Claims
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1. An immobilized material comprising a biologically active enzyme bound by covalent bonding with a crosslinking agent or condensing agent onto a carrier which is a water-insoluble acrylonitrile polymer containing from 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer and having a porous structure containing a number of micro-pores with an average size of from 40 to 9000 A, said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- M to 1000 μ
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14. A process for preparing an immobilized material which comprises binding a carrier of a water-insoluble acrylonitrile polymer containing from 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer with a biologically active enzyme by covalent bonding with a crosslinking agent or condensing agent in an inert medium;
said polymer having a porous structure containing a number of micro-pores with an average size of 40 to 9000 A, said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
- M to 1000 μ
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26. A device for promoting an enzymatic reaction comprising a means containing an immobilized material having a biologically active enzyme bound by covalent bonding with a crosslinking agent or condensing agent to a carrier comprising a water-insoluble acrylonitrile polymer containing amino groups and having a porous structure having a number of micro-pores with an average pore size of 40-9000 A, said polymer containing 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer and said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert nonsolvent with lithium aluminum hydride, a storage means containing a liquid substrate on which said biologically active enzyme has an activity and means for transporting at least a portion of the substrate from said storage means to said immobilized material. - View Dependent Claims (27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
- M to 1000 μ
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44. A method for effecting an enzymatic reaction by use of an immobilized material, which comprises reacting an immobilized material comprising a biologically active enzyme bound to a carrier by covalent bonding, with a crosslinking agent or condensing agent, said carrier comprising an acrylonitrile polymer containing amino groups and having a porous structure having a number of micro-pores with an average pore size of 40-9000 A, said polymer containing 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer and acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride, with a substrate on which said biologically active enzyme has activity. - View Dependent Claims (45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57)
- M to 1000 μ
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58. A process for preparation of 6-aminopenicillanic acid which comprises enzymatically deacylating a benzylpenicillin or alkaline salt thereof by using immobilized penicillin acylase bound to a carrier of water-insoluble acrylonitrile polymer containing from 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer, with the penicillin acylase bound by covalent bonding with a crosslinking agent or condensing agent in an inert medium, said polymer having a porous structure containing a number of micro-pores with an average size of 40 to 9000 A, said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride. - View Dependent Claims (59, 60, 61, 62, 63, 64)
- M to 1000 μ
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65. A process for preparation of 7-amino-desacetoxy cephalosporanic acid which comprises enzymatically deacylating a 7-phenylacetamidodesacetoxy cephalosporanic acid by using immobilized acylase bound to a carrier of water-insoluble acrylonitrile polymer containing from 20 μ
- M to 1000 μ
M of amino groups per gram of the polymer, with the acylase bound by covalent bonding with a crosslinking agent or condensing agent in an inert medium, said polymer having a porous structure containing a number of micro-pores with an average size of 40 to 9000 A, said acrylonitrile polymer being produced by effecting reduction of an acrylonitrile polymer having said porous structure in an inert non-solvent with lithium aluminum hydride. - View Dependent Claims (66, 67, 68, 69, 70, 71)
- M to 1000 μ
Specification