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Vectors for the insertion therein of foreign DNA fragments, according to any translation phase

  • US 4,371,625 A
  • Filed: 11/13/1979
  • Issued: 02/01/1983
  • Est. Priority Date: 11/13/1978
  • Status: Expired due to Term
First Claim
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1. A composition of matter for the introduction of foreign DNA into a host whereby the expression of a protein having a desired amino acid sequence is provided, said composition of matter comprising the following three structurally interrelated vectors:

  • (A) a first vector which comprises;

    (i) a preselected bacterial promoter in association with a preselected bacterial gene or part thereof, said preselected bacterial gene or part thereof comprising a translation initiation point,(ii) a single recognition site for a preselected restriction endonuclease, said site being in association with the preselected bacterial gene or part thereof, and(iii) a plurality of nucleotide base pair triplets between the translation initiation point of the gene or part thereof and the single restriction endonuclease recognition site associated with the gene or part thereof, wherein the plurality of nucleotide base pair triplets does not contain a nonsense codon, and(B) a second vector which comprises(i) (A) (i),(ii) (A) (ii) and(iii) the plurality of nucleotide base pair triplets plus two additional nucleotide base pairs between the translation initiation point of the gene or part thereof and the single restriction endonuclease recognition site associated with the gene or part thereof, wherein the plurality of nucleotide base pair triplets does not contain a nonsense codon, and(C) a third vector which comprises;

    (i) (A) (i),(ii) (A) (ii) and(iii) the plurality of nucleotide base pair triplets plus 1 or 4 additional nucleotide base pairs between the translation initiation point of the gene or part thereof and the single restriction endonuclease recognition site associated with the gene or part thereof, wherein the plurality of nucleotide base pair triplets does not contain a nonsense codon.

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