Method and device for analysis with color identification test paper

• US 4,373,818 A
• Filed: 01/23/1979
• Issued: 02/15/1983
• Est. Priority Date: 01/25/1978
• Status: Expired due to Term

• Current Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Original Assignee: Kabushiki Kaisha Kyoto Daiichi Kagaku
• Inventors: Yamamoto, Hiroshi, Furutani, Yoshikazu
• Primary Examiner(s): ARNOLD, BRUCE
• Application Number:
  US06/005,859
• Time in Patent Office: 1,484 Days
• Field of Search: 356/445-448, 356/408, 356/416, 356/418, 356/421, 356/425, 356/402, 356/415, 356/422, 356/423, 356/409, 356/412
• US Class Current: 356/408
• CPC Class Codes: G01N 21/4738 Diffuse reflection precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration, e.g. also for testing fluids, fibrous materials
• CPC Class Codes: G01N 2201/065 Integrating spheres

Abstract

A method and apparatus are disclosed for an analyzer which measures concentration of a liquid specimen by monitoring the coloration of test paper which is either dipped into the specimen or is otherwise painted with it. In the method, inner stray rays produced by a light source which do not reach the test paper are experimentally monitored, so that the effect of such inner stray rays upon the accuracy of the test paper analysis is eliminated. Additionally, a method of relating relative reflectivity of the test paper to the concentration of the specimen is disclosed, in which method the calibration curve is modeled as a section of a hyperbola. In the apparatus, a microcomputer performs the evaluation and the detection of the coloration of the test paper and the compensation for various sources of instrumental error takes place electronically.

Claims

1. A method for analyzing concentration of substance under analysis in an electronic analytical instrument having a light source, which method determines the concentration by measuring, at at least one wavelength of interest, light reflected from a test piece of paper bearing the substance and light reflected from a standard having a known reflectivity, and by forming a ratio of these measurements, which ratio is corrected for light source intensity variations and for inner stray rays, said ratio being termed the relative reflectivity of the test piece, said method comprising the steps of:

determining a constant of the instrument useful in correcting the instrument for the effects of light source intensity variations and inner stray rays;

storing said constant electronically;

using the stored constant to determine a value proportional to the detected inner stray rays; and

using said value to determine the relative reflectivity of the test piece.

2. The method defined by claim 1, further including the step of calibrating the instrument by using two standards having a known relative reflectivity and adjusting the instrument to indicate said known relative reflectivity while comparing light reflected from one standard with light reflected from the other standard.

3. The method defined by claim 1 further comprising the step of calibrating the instrument by:

establishing a standard quantity by intermittently illuminating the standard with light and subtracting light output detected during a period of non-illumination of the standard from light output detected during a period of illumination of the standard, whereby a correction for dark current, outer stray rays and amplifier offset is accomplished;

establishing an inner stray rays constant from light output detected during illumination of a totally non-reflective standard, whereby a correction for inner stray rays is accomplished;

establishing a test quantity by intermittently illuminating the test piece with light and subtracting light output detected during a period of non-illumination of the test piece from light output detected during a period of illumination of the test piece; and

computing relative reflectivity of the test piece using the standard quantity, the test quantity, and the constant.

4. The method defined by claim 2 or 3, further comprising the step of converting the relative reflectivity of the test piece into concentration of the substance.

5. The method defined by claim 4, wherein said step of converting is carried out in accordance with an equation in which ##EQU7## where Y=concentration of the substance

r=the relative reflectivity of the test piece and

a, b, and C are empirically derived constants.

6. An electronic apparatus for analyzing concentration of a substance under analysis and which measures the concentration by measuring light reflected from a test piece of paper bearing the substance and light reflected from a standard having a known reflectivity by forming a ratio which is corrected for light source intensity variations and inner stray rays, said ratio being termed the relative reflectivity of the test piece, said apparatus comprising:

optical means for irradiating an object with light of a predetermined wavelength;

a second means for producing a constant signal which corresponds to a constant of the instrument useful in determining inner stray rays present during irradiation of the object;

means for electronically storing the constant signal;

means for detecting light reflected from the object during irradiation and for producing a first signal and a second signal which correspond, respectively, to light reflected off the test piece and light reflected off the standard;

means for deriving from the stored constant signal a correction signal representing inner stray rays present during irradiation of the object; and

a fourth means for determining the relative reflectivity of the test piece in response to the correction signal, the first signal and the second signal, in a manner such that the relative reflectivity of the test piece is corrected for effects of light source intensity variations and inner stray rays.

7. The apparatus defined by claim 6, wherein the fourth means comprises computer means.

8. The apparatus defined by claim 7, wherein the correction signal, the first signal and the second signal are all analog signals, wherein the computer means is a digital computer means, and wherein the fourth means includes an analog to digital converter which converts the correction signal, the first signal and the second signal into digital signals for application to said computer means.

9. The apparatus defined by claim 8, wherein the computer means includes means for storing the constant signal prior to detecting light reflected from the test piece.

10. The apparatus defined by claim 6, further comprising a means for converting the relative reflectivity of the test piece into a corresponding concentration of the substance.

11. The apparatus defined by claim 10, further comprising a means for storing the corresponding concentration of the substance as a concentration signal.

12. The apparatus defined by claim 11, wherein the concentration signal is derived in accordance with an equation in which:

##EQU8## where;

Y=concentration of the substance

r=the relative reflectivity of the test piece and

a, b and C are empirically derived constants.

13. The apparatus defined by claim 12, wherein the concentration signal is a digital signal.

14. The apparatus defined by claim 10, wherein the fourth means comprises a computer.

15. The apparatus defined by claim 14, wherein the correction signal, the first signal and the second signal are all analog signals, wherein the computer is a digital computer, and wherein the fourth means includes an analog to digital converter which converts the correction signal, the first signal and the second signal into digital signals for application to the computer.

16. The apparatus defined by claim 6, wherein the optical means includes a means for interrupting irradiation of the object.