Oxygen stabilized enzyme electrode

• US 4,374,013 A
• Filed: 03/03/1981
• Issued: 02/15/1983
• Est. Priority Date: 03/05/1980
• Status: Expired due to Fees

• Current Assignee: Sven-Olof Enfors
• Original Assignee: Sven-Olof Enfors
• Inventors: Enfors, Sven-Olof
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/239,979
• Time in Patent Office: 714 Days
• Field of Search: 435/817, 204/1 E, 204/195 P, 204/195 B
• US Class Current: 204/403.12
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An enzyme electrode based on an oxygen electrode and an oxygen consuming enzyme is described. The oxygen demand of the electrode is satisfied through an electrolytical oxygen production by means of an anode located close to the enzyme and the sensitive surface of the oxygen electrode. The deviation from a reference value, which could be constant or be formed by the signal from an enzymatically passive oxygen electrode, of the oxygen electrode signal, is used to control the electrolysis current in order to keep the oxygen activity in the enzyme electrode constant. The electrolysis current is used as a measure of the concentration of the substrate of the enzyme.

Claims

1. Enzyme electrode for the analysis of a substrate in a sample comprising:

(a) an oxygen electrode on which or close to the sensitive surface of which at least one enzyme is immobilized, said oxygen electrode producing a signal responsive to the consumption of oxygen by the reaction between the enzyme and the substrate,

(b) means for producing a reference signal,

(c) means for electrolytically producing oxygen by the electrolysis of water, comprising an anode located close to the at least one enzyme, and a cathode,

(d) means for controlling the electrolysis current between the anode and cathode for producing oxygen in response to the difference between the signal from the oxygen electrode and the reference signal so that the limiting parameter for the enzyme reaction is the enzyme activity, and

(e) means for measuring the electrolysis current.

2. Enzyme electrode as claimed in claim 1 wherein said at least one enzyme is immobilized on said electrolysis anode.

3. Enzyme electrode as claimed in claim 2 wherein the electrolysis anode is in contact with a gas permeable membrane of said oxygen electrode.

4. Enzyme electrode as claimed in claim 3 wherein the electrolysis anode, the at least one immobilized enzyme, and the oxygen electrode are within a tube, said tube having a membrane which is permeable to said substrate, said electrolysis cathode being in electrolytical contact with the sample.

5. Enzyme electrode as claimed in claim 4 wherein the electrolysis cathode is located outside of the tube.

6. Enzyme electrode as claimed in claim 1 wherein said means for controlling the electrolysis current comprises an amplifier for amplifying the difference in said signals, a PI regulator for the amplified signal, and a current regulator.

7. Enzyme electrode as claimed in claim 1 wherein the means for producing the reference signal is another oxygen electrode which is in said sample.

8. Enzyme electrode as claimed in claim 1 wherein the reference signal is of constant value.

9. Enzyme electrode as claimed in claim 1, 7, or 8 wherein the enzyme at least partly consists of an oxidase or an oxygenase.

10. Enzyme electrode as claimed in claim 5 wherein said means for controlling the electrolysis current maintains a constant oxygen activity in the enzyme, the electrolysis current being controlled so as to reduce said difference towards zero.