Testing reagent

• US 4,374,041 A
• Filed: 04/24/1978
• Issued: 02/15/1983
• Est. Priority Date: 03/01/1974
• Status: Expired due to Term

• Current Assignee: Esa S.R.O. (Logisteed Limited)
• Original Assignee: Environmental Sciences Associates Incorporated
• Inventors: Matson, Wayne R.
• Primary Examiner(s): Barr, J. L.
• Application Number:
  US05/899,389
• Time in Patent Office: 1,758 Days
• Field of Search: 252/408, 252/500, 252/519, 252/79.2, 204/1 T, 23/230 B, 23/230 R, 23/230 M, 23/305 R, 23/230 HC
• US Class Current: 205/789.5
• CPC Class Codes: G01N 27/42 Measuring deposition or liberation of materials from an electrolyte; Coulometry, i.e. measuring coulomb-equivalent of material in an electrolyte
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage

Abstract

A reagent composition particularly adapted for use in treating organic samples suspected of containing chemically-bound heavy metals preparatory to electrically detecting and measuring the heavy metal content of the samples. The composition includes an electrolyte vehicle which is essentially free of the heavy metal being detected and measured, a measured quantity of at least one metal ion-containing reagent dissolved therein, said metal ion (1) being adapted to displace the heavy metal from its organic chemical bonding and (2) remaining in electrolytic solution at the electrolytic deposition potentials of the heavy metals being detected and measured, a dispersing agent, and a hydrogen ion source for adjusting the pH of said composition to pH about 1.

Claims

1. A reagent composition for treating an organic sample suspected of containing chemically-bound heavy metal preparatory to electrochemically detecting and measuring the heavy metal content of said organic sample, said composition comprising in combination:

an electrolytic vehicle which is essentially free of said heavy metal being detected and measured,

a measured quantity of at least one metal ion-containing reagent dissolved therein, said metal ion (1) being capable of displacing said heavy metal from its organic chemical bonding while (2) remaining in electrolytic solution at the electrolytic deposition potentials of the heavy metals being detected and measured,

a dispersing agent, and

a hydrogen ion source for adjusting the pH of said composition to pH about 1.

2. The composition of claim 1, wherein said organic sample comprises a biological sample and the heavy metal being detected and measured is lead, and wherein said metal ion-containing reagent comprises chromic ion or calcium ion.

3. The composition of claim 1, wherein said metal ion-containing reagent comprises a mixture of chromic ion and calcium ion.

4. The composition of claim 1, wherein each ingredient of said composition in measured quantity is prepackaged and contained within a sealed sample holder which is also essentially free of the heavy metals to be detected and measured.

6. The composition of claim 1, wherein said organic sample comprises a biological sample selected from the group consisting of blood, tissue, and food.

7. The composition of claim 1, wherein said organic sample comprises a non-biological sample selected from the group consisting of paint and gasoline.

8. The composition of claim 1, wherein said organic sample is suspected of containing a heavy-metal selected from the group consisting of lead, cadmium, copper, zinc, thallium, silver, gold, bismuth and mixtures of two or more of said group, and said metal ion-containing reagent comprises chromic ion or calcium ion.

9. The composition of claim 1, wherein said organic sample is suspected of containing a heavy metal selected from the group consisting of lead, cadmium, copper, zinc, thallium, silver, gold, bismuth and mixtures of two or more of said group, and said metal ion-containing reagent comprises a mixture of chromic ion and calcium ion.

10. The composition of claim 1, wherein said dispersing agent comprises a non-ionic alcohol.

5. A composition for treating a human blood sample preparatory to electrochemically detecting and measuring the lead content of said sample, said composition comprising:

an electrolytic solution containing a dissovled chromic compound and a dissolved calcium compound, the chromic and calcium ions together being present in concentration of about 0.12 F, dissolved mercuric chloride in a concentration of about 0.000225 F, hydrazine in a concentration of about 0.019 F, phosphoris acid in a concentration of about 0.01 F, a dispersing agent, and a source of hydrogen ions to bring said composition to pH about 1.

11. A composition for treating a gasoline sample preparatory to electrochemically detecting and measuring the lead content of said sample, said composition comprising:

an electrolytic solution containing dissolved sodium ion in concentration of about 0.1 molar, dissolved bromide ion in concentration of about 0.01 molar,

a dispersing agent, and

a hydrogen ion source for adjusting the pH of said composition to pH about 1.

12. The composition of claim 11, wherein said dispersing agent comprises a non-ionic alcohol.