Method and apparatus for on-column detection in liquid chromatography

• US 4,375,163 A
• Filed: 01/08/1981
• Issued: 03/01/1983
• Est. Priority Date: 01/08/1981
• Status: Expired due to Fees

• Current Assignee: Varian Associates Palo Alto CA
• Original Assignee: Varian Associates, Inc.
• Inventors: Yang, Frank J.
• Primary Examiner(s): Kazenske, Edward R.
• Assistant Examiner(s): ROSKOS, JOSEPH
• Application Number:
  US06/223,445
• Time in Patent Office: 782 Days
• Field of Search: 73/61.1 C, 250/373, 250/372, 356/410, 356/411, 210/198.2, 210/656
• US Class Current: 73/61.53
• CPC Class Codes: G01N 30/6078 Capillaries
• CPC Class Codes: G01N 30/6082 transparent to radiation
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J

Abstract

On-column detection by an optical detector is accomplished with a flexible silica column. The flexible fused silica column has an inner diameter less than 500 μm, an external protective coating with a stripped portion near the end of the column. The stripped portion is placed in the working path of an optical detector in order to detect and resolve the sample.

Claims

1. A method for high resolution on-column detection in high resolution liquid chromatography, comprising:

providing a fused silica microcapillary column having an inner diameter less than 500 μ

m;

activating the interior of said column with a chromatographically active material;

applying a protective coating to the exterior of said column;

removing a segment of said protective coating to expose the underlying segment of said fused silica column;

providing an optical chromatographic detector;

positioning said exposed segment of said column in the optical path of said detector;

flowing a sample containing species to be identified through said column; and

detecting separated species as they flow through said exposed segment of said column.

2. A method for on-column detection in accordance with claim 1 wherein said step of activating the interior of said column is accomplished by the step of packing said column with a chromatographically active material and wherein said packing is removed within the volume of said exposed segment.

3. A method for on-column detection in accordance with claim 1 wherein said step of activating the interior of said column is accomplished by the step of coating the inner wall of said column with a chromatographically active material.

4. A method for on-column detection in accordance with claim 3 in combustion with the step of removing said chromatographically active material along said exposed segment.

5. A method for on-column detection in accordance with claim 1 wherein said step of providing a chromatographic detector is accomplished by the step of providing an ultraviolet absorbance detector.

6. Apparatus for on-column detection in high resolution liquid chromatography comprising:

a flexible fused silica column having an inner diameter less than 500 μ

m, said column being chromatographically activated and also having an external protective coating, said external coating having a segment exposed along the end of its length; and

an optical chromatographic detector configured to receive said column so that said exposed segment lies in the optical detection path of said detector.

7. Apparatus for on-column detection in accordance with claim 6 wherein said exposed segment is less than one centimeter in length.

8. Apparatus for on-column detection in accordance with claim 6 wherein said optical chromatographic detector is an ultraviolet detector.

9. Apparatus for on-column detection in accordance with claim 7 wherein said column is packed with a chromatographically active material.

10. Apparatus for on-column detection in accordance with claim 7 wherein the inner surface of said column is coated with a chromatographically active material.