Molecule selective sensor for industrial use and procedure for its preparation

• US 4,375,399 A
• Filed: 12/19/1980
• Issued: 03/01/1983
• Est. Priority Date: 09/08/1978
• Status: Expired due to Fees

• Current Assignee: Radelkis Elektrokemiai Miszergyarto Szovetkezet
• Original Assignee: Radelkis Elektrokemiai Miszergyarto Szovetkezet
• Inventors: Pungor, Erno, Nagy, Geza, Havas, Jeno, Porjesz, Emma
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/218,102
• Time in Patent Office: 802 Days
• Field of Search: 204/1 E, 204/195 B, 204/195 P, 435/817, 435/288, 435/175, 435/177, 435/180, 435/14, 435/25
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A molecule selective sensor having two permeable membranes in contacting relationship with each other, one membrane being a protein membrane found in nature, as for example, hog intestine membrane, air bladder of fish, epidermal section of mammals and reptiles, etc. and the other membrane being the reaction product of acrylamide, polyvinylalcohol, an enzyme, bovine albumin and glutaric aldehyde.

Claims

1. A process for producing an enzymatically active membrane which consists in mixing together 1-20 weight percent of acrylamide, 1-20 weight percent of polyvinylalcohol, 0.01-10 weight percent of bovine albumin, 0.0001-5 weight percent of an enzyme, 0.1-5 weight percent of glutaric aldehyde, a polymerization initiating amount of potassium persulfate and the remainder to 100 weight percent of distilled water, spreading the mixture on a protein membrane found in nature to form a thin sheet and allowing the mixture to react for 12 hours at room temperature.

2. A process as claimed in claim 1 wherein the mixture contains in addition 0.1-5 weight percent of N,N'"'"'-methylene-bis-acrylamide.

3. A hollow molecule-selective sensor having a sensing electrode and a reference electrode and two permeable membranes in contacting relationship with each other, one of said membranes being a protein membrane found in nature and the other of said membranes being a self-supporting enzymatically active membrane prepared according to the process of claim 1.

4. A sensor as claimed in claim 3 wherein the said protein membrane found in nature is selected from the group consisting of hog intestine membrane, air bladder of fish, epidermal section of a mammal, epidermal section of a reptile and corneal section.

5. A sensor as claimed in 3 wherein the self-supporting enzymatically active membrane comprises two or more enzymes.