Coenzyme immobilized electrode

• US 4,376,689 A
• Filed: 11/13/1981
• Issued: 03/15/1983
• Est. Priority Date: 04/21/1978
• Status: Expired due to Term

• Current Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Matsushita Electric Industrial Company Limited (Panasonic Holdings Corporation)
• Inventors: Iijima, Takashi, Nankai, Shiro, Nakamura, Kenichi
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US06/321,326
• Time in Patent Office: 487 Days
• Field of Search: 204/195 B, 204/1 E, 204/195 R, 435/4, 435/288, 435/817
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Improvements in a coenzyme electrode used to electrochemically measure the activity of enzyme or substrate concentration of the enzyme easily. The coenzyme is immobilized, without using a semipermeable membrane, on the electron collector directly with the chemical bond, particularly the covalent bond, whereby the activity of the oxide-reductase requiring the coenzyme can be measured. In addition, the oxide-reductase requiring the coenzyme is also immobilized together with such immobilized coenzyme to improve the characteristics of the conventional enzyme-coenzyme immobilized electrode.

Claims

1. A process for preparing a coenzyme immobilized electrode, which comprises (1) providing a carrier adapted to immobilize, through covalent bonding, a coenzyme of oxidoreductase, said coenzyme being selected from the group consisting of NAD and NADP, (2) immobilizing said coenzyme on said carrier, (3) mixing said carrier, having said coenzyme immobilized thereon through covalent bonding, with an electron collector, and (4) press-molding the resultant mixture to form a coenzyme electrode.

2. A coenzyme immobilized electrode comprising at least a coenzyme of oxidoreductase, an electron collector and a carrier, said coenzyme, which is selected from the group consisting of NAD and NADP, being immobilized through covalent bonding on said carrier which is mixed with said electron collector, said immobilized coenzyme being in electrical contact with said electron collector.

3. A coenzyme immobilized electrode in accordance with claim 2, wherein said carrier is selected from the group consisting of albumin, agarose, cellulose, silica glass, alumina, silica alumina and zeolite.