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Heterogenous chemiluminescent specific binding assay

  • US 4,380,580 A
  • Filed: 06/21/1979
  • Issued: 04/19/1983
  • Est. Priority Date: 04/10/1978
  • Status: Expired due to Term
First Claim
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1. In a heterogeneous specific binding assay method for determining a ligand in a liquid medium, which method comprises the steps of:

  • (a) contacting said liquid medium with reagent means including a labeled conjugate comprising a specific binding substance coupled to a labeling substance, said reagent means and the ligand forming a binding reaction system producing a bound-phase and a free-phase of the labeled conjugate;

    (b) separating said bound-phase and said free-phase; and

    (c) determining said labeling substance in said bound-phase or said free-phase as a function of said ligand in said liquid medium,the improvement wherein said labeling substance is a chemiluminescent reactant and wherein said chemiluminescent label is determined in said bound-phase or said free-phase by forming the chemiluminescent reaction therein and measuring the light produced.

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