Automatic enzyme immunoassay apparatus

• US 4,383,041 A
• Filed: 10/21/1981
• Issued: 05/10/1983
• Est. Priority Date: 10/28/1980
• Status: Expired due to Fees

• Current Assignee: Fujirebio Kabushiki Kaisha
• Original Assignee: Fujizoki Pharmaceutical Company Limited (H.U. Group Holdings, Inc.)
• Inventors: Hisada, Hideho, Fujihara, Mikiharu, Shirane, Hideo, Kutsusawa, Tadashi
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/313,682
• Time in Patent Office: 566 Days
• Field of Search: 422/63, 422/65, 435/288, 436/808
• US Class Current: 435/287.2
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

An apparatus for automatically performing a sandwich-type enzyme immunoassay. The apparatus includes a rack for holding test tubes. In the test tubes are beads which provide surfaces for the immuochemical reactions. The rack can be moved in lengthwise and transverse directions. Nozzles for supplying and withdrawing liquids from the test tubes are included. The bead in one tube can be transferred to a second tube. The absorbance of a final liquid is measured spectrophotometrically, and the results are recorded by a printer.

Claims

1. An automatic enzyme immunoassay apparatus comprising:

means for moving a rack holding test tubes containing beads therein, in a lengthwise direction, said rack being held in a cage;

means for moving said rack in a direction transverse to said lengthwise direction;

means for inserting both a suction nozzle and a cleaning liquid injection nozzle into any of or into a plurality of said test tubes to inject cleaning liquid into said test tube after withdrawing liquid by suction from said test tube and discarding the withdrawn liquid;

means for inserting a reaction liquid injection nozzle into said test tube and injecting a reaction liquid into said test tube;

means for vibrating said test tube;

means for inserting an attraction nozzle into said test tube and pulling said bead out of said test tube, while attracting said bead thereto, and letting said bead fall into a second test tube;

means for inserting a substrate injection nozzle and a reaction termination liquid injection nozzle, and injecting a reaction termination liquid into said second test tube after injecting a substrate;

means for inserting a nozzle associated with a measuring device into said second test tube and transporting the liquid in said test tube to a measurement device;

a spectrophotometer for measuring the absorbance of said liquid; and

a printer for recording the measured value of the absorbance.