System for determining the concentration of an L-amino acid in fermentation
First Claim
1. A microbial electrode for determining the concentration of an L-amino acid in a culture medium or a cultured broth solution, which comprises:
- a carbon dioxide gas sensitive electrode having a carbon dioxide permeable membrane;
anaerobic containing means containing said membrane and capable of containing a portion of said broth or mixture;
a microorganism capable of anaerobically decarboxylating said L-amino acid immobilized on said membrane; and
inert gas supplying means injectably attached to said containing means.
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Abstract
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an oxygen-sensitive electrode or a carbon dioxide gas-sensitive electrode combined with fixed microbial cells of a microorganism capable of assimilating said carbon source or decarboxylating said L-amino acid with a sample solution and sensing electrically the rate of current decrease which is caused by the consumed oxygen in the solution in proportion to the concentration of carbon source; or the electric motive force which is caused by the liberation of carbon dioxide in the sample solution in proportion to the concentration of L-amino acid. Microbial electrodes and systems useful in carrying out the aforementioned method are disclosed.
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Citations
7 Claims
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1. A microbial electrode for determining the concentration of an L-amino acid in a culture medium or a cultured broth solution, which comprises:
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a carbon dioxide gas sensitive electrode having a carbon dioxide permeable membrane; anaerobic containing means containing said membrane and capable of containing a portion of said broth or mixture; a microorganism capable of anaerobically decarboxylating said L-amino acid immobilized on said membrane; and inert gas supplying means injectably attached to said containing means. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A system for determining the concentration of an L-amino acid in a culture medium or cultured broth, which comprises:
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(a) a carbon dioxide gas sensitive electrode having a carbon dioxide permeable membrane; (b) anaerobic containing means containing said membrane; (c) a microorganism capable of anaerobically decarboxylating said L-amino acid immobilized on said membrane; (d) anaerobic conveying means for anaerobically conveying a mixture of a sample of said L-amino acid and a buffered carrier solution saturated with an inactive gas to said containing means; (e) sample supplying means for supplying a sample of said L-amino acid to said conveying means; (f) buffered carrier solution supplying means for supplying a buffered carrier solution to said conveying means; (g) inactive gas supplying means for supplying inactive gas to said conveying means; and (h) measuring means or recording means electrically connected to said carbon dioxide electrode.
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Specification