Double tagged immunoassay
First Claim
1. In a method of performing an immunoassay in which a receptor ligand is contacted with a test ligand which binds immunologically with the receptor ligand and the receptor and/or test ligands are contacted with an assay ligand which is capable of immunologically binding to the receptor and/or test ligand the improvement which comprises employing a receptor ligand containing a tagging constituent which is independent of the tagging constituent on the test ligand.
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Accused Products
Abstract
Immunoassays are performed with two different ligands tagged with two different tagging constituents which are independent of each other and the two tagged ligands are immunologically bound together. The two different ligands may be detected independently through their independent tagging constituents for quality control, internal calibration (standardization), determination of viability and shelf life and the like.
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Citations
6 Claims
- 1. In a method of performing an immunoassay in which a receptor ligand is contacted with a test ligand which binds immunologically with the receptor ligand and the receptor and/or test ligands are contacted with an assay ligand which is capable of immunologically binding to the receptor and/or test ligand the improvement which comprises employing a receptor ligand containing a tagging constituent which is independent of the tagging constituent on the test ligand.
- 3. In a method of performing an immunoassay in which a receptor ligand is contacted with a test ligand which binds immunologically with the receptor ligand and the receptor and/or test ligands are contacted with an assay ligand which is capable of immunologically binding to the receptor and/or test ligand the improvement which comprises employing a receptor ligand containing a tagging constituent which is independent of the tagging constituent on the test ligand, and measuring the quantity of assay ligand by measuring the quantities of tagging constituents on both the receptor ligand and test ligand while said receptor and test ligand are immunologically bound together.
Specification