Affinity chromatography separation process

• US 4,385,991 A
• Filed: 10/01/1980
• Issued: 05/31/1983
• Est. Priority Date: 12/15/1976
• Status: Expired due to Term

• Current Assignee: AEA Technology PLC (AEA Technology Group PLC)
• Original Assignee: United Kingdom Atomic Energy Authority
• Inventors: Rosevear, Alan, Mattock, Patrick
• Primary Examiner(s): Adee, John
• Application Number:
  US06/192,831
• Time in Patent Office: 972 Days
• Field of Search: 210/635, 210/656, 210/198.2, 252/426, 252/428
• US Class Current: 210/635
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01J 20/28019 Spherical, ellipsoidal or cylindrical
• CPC Class Codes: B01J 20/28047 Gels
• CPC Class Codes: B01J 20/28097 being coated, filled or plugged with specific compounds
• CPC Class Codes: B01J 20/291 Gel sorbents
• CPC Class Codes: B01J 2220/54 Sorbents specially adapted for analytical or investigative chromatography
• CPC Class Codes: C07K 14/765 Serum albumin, e.g. HSA

Abstract

The invention discloses a process for the selective separation of a chemical component from a fluid substance which comprises contacting the fluid substance with a composite material having affinity chromatography properties, said composite material comprising an affinity chromatography agent retained within the pore structure of a porous rigid support material and said affinity chromatography agent being capable of selectively sorbing the chemical component from the fluid substance.

Claims

1. A chromatography process for the selective separation of a chemical component from a fluid substance which comprises contacting the fluid substance with a composite material having affinity chromatography properties, said composite material comprising an inorganic porous rigid support material having within the pores thereof a deformable gel to which is coupled an affinity chromatography ligand being capable of selectively sorbing the chemical component from the fluid substance, the outside surface of said support being substantially free of said deformable gel.

2. A process as claimed in claim 1 wherein the porous rigid support is in the form of discrete porous particles.

3. A process as claimed in claim 2 wherein the discrete porous particles are those prepared by a method for producing an inorganic material having interconnected porosity throughout the material for the selective retention of predetermined molecules from a fluid substance containing said molecules including the steps of mixing a finely divided, substantially insoluble, sorptive, inorganic material, capable of sorbing the molecules, with a solid fugitive additive to form a mixture, including in the mixture a solvent to dissolve fugitive additive in the solvent, said solvent, forming discrete particles from the mixture, and heating the particles to remove solvent and fugitive additive to produce discrete particles of said inorganic material having an interconnected pore structure throughout said discrete particles providing an extended surface area, the pore size being such as will allow said predetermined molecules in said fluid substance to permeate the inorganic particles and be sorbed, said inorganic material being substantially unaffected by said heating utilized to effect removal of solvent and fugitive additive.

4. A process as claimed in claim 2 wherein the discrete porous particles are discrete particles of a natural earth.

5. A process as claimed in claim 1 wherein a component of the deformable gel is an affinity chromatography agent comprising a group specific ligand capable of interacting specifically with the nucleotide binding sites of an enzyme.

6. A process as claimed in claim 1 wherein a component of the deformable gel is an affinity chromatography agent comprising an affinity chromatography ligand capable of retaining proteins from human plasma.

7. A process as claimed in claim 1 wherein a component of the deformable gel is a polysaccharide gel or a synthetic polymer gel.

8. A process as claimed in claim 7 wherein the polysaccharide gel is an agarose or a cellulose gel.

9. A process as claimed in claim 7 wherein the synthetic polymer gel is a polymer of an acrylate or a polyvinyl alcohol gel.

10. A process as claimed in claim 1 wherein the composite material is in the form of substantially spherical particles.

11. A process as claimed in claim 1 wherein the chemical component is albumin.

12. A process as claimed in claim 1 wherein the chemical component is subsequently recovered from the composite material by contacting the composite material with a solution of a reagent capable of desorbing the chemical component therefrom.

13. A chemical component whenever separated from a fluid substance by a process as claimed in claim 1.