Method and apparatus for chemical analysis using reactive reagents dispersed in soluble film

• US 4,387,164 A
• Filed: 11/05/1980
• Issued: 06/07/1983
• Est. Priority Date: 11/05/1980
• Status: Expired due to Term

• Current Assignee: FMC Corporation
• Original Assignee: FMC Corporation
• Inventors: Hevey, Richard C., Forget, Ronald D.
• Primary Examiner(s): Marantz, Sidney
• Application Number:
  US06/204,112
• Time in Patent Office: 944 Days
• Field of Search: 422/56, 422/72, 436/45, 436/165, 436/166
• US Class Current: 436/45
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/70 involving creatine or creatinine
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor

Abstract

Automated chemical analytical method and device in which a precise measured quantity of reagent is combined with an assay medium. Prior to such delivery, the reagent, being water-soluble or dispersible, is contained within carrier solid organic binder which is fixed within and constitutes part of the device. Upon addition of an aqueous medium, the carrier binder is dissolved or dispersed, the protected precise measured quantity of reagent is concomitantly released and dissolved.

Claims

1. In a method of chemically analyzing an assay medium in which at least one solid or liquid reagent is supplied to a well and subsequently and automatically the reagent and an assay medium are mixed for reaction, the reaction detected, and the reaction data presented, the improvement comprising supplying reagents to such one well by combining therein an aqueous medium and at least one element consisting essentially of carrier solid organic binder which is soluble or dispersible in water and in which is dispersed at separate locations thereof a precise, measured quantity of each of at least two water-soluble or dispersible reagents which are reactive with each other whereby the protected, measured quantities of reagents dispersed within the solid organic binder are completely dissolved or dispersed concomitantly as the binder is dissolved or dispersed by the aqueous medium, mixed with each other, and reacted prior to mixing with the assay medium.

2. In a device for use with an apparatus for automatically chemically analyzing an assay medium, said device having a well into which is supplied at least one solid or liquid reagent which, when said device is used with an analyzing apparatus, is automatically mixed with an assay medium for reaction, the reaction detected, and the reaction data presented, the improvement comprising an element fixed within the well of said device in position to be contacted by an aqueous medium to be supplied thereto, said element consisting essentially of carrier solid organic binder which is soluble or dispersible in water and which contains therein at separate locations thereof a measured quantity of each of at least two water-soluble or dispersible reagents which are reactive with each other whereby the protected and unreacted reagents are completely dissolved or dispersed and react with each other concomitantly as the carrier binder is dissolved or dispersed upon supplying aqueous medium into the well of such device.

3. In a device as defined in claim 2 wherein said element is in the form of a solid film fixed within the well of said device by adhesion.

4. In a device as defined in claim 3 wherein said element is in the form of a solid film and is adhered within the well of said device by being cast therein a flowable condition and solidifying the same.