Method and apparatus for dielectrophoretic manipulation of chemical species

• US 4,390,403 A
• Filed: 07/24/1981
• Issued: 06/28/1983
• Est. Priority Date: 07/24/1981
• Status: Expired due to Fees

• Current Assignee: Batchelder, J. Samuel
• Original Assignee: Batchelder, J. Samuel
• Inventors: Batchelder, J. Samuel
• Primary Examiner(s): Demers, Arthur P.
• Application Number:
  US06/286,387
• Time in Patent Office: 704 Days
• Field of Search: 204/180 P, 204/180 R
• US Class Current: 204/547
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 3/502792 for moving individual droplets on a plate, e.g. by locally altering surface tension
• CPC Class Codes: B03C 5/022 Non-uniform field separators

Abstract

The present invention provides method and apparatus for manipulating one or more chemicals within a reaction chamber or housing by dielectrophoretic forces. At least two materials, one of which is a chemical to be manipulated, are provided within the housing. The materials have different dielectric constants. A non-uniform electrical field is applied to the materials within the housing and, as a result of dielectrophoretic forces generated by the applied field, the relative positions of the materials are varied. Accordingly, a chemical can be selectively manipulated to different positions within the housing as, for example, to a catalyst or chemical analyzer located within the housing. The present apparatus may also be used to simultaneously manipulate more than one chemical to mix, or induce a chemical reaction, between the different chemicals in the housing.

Claims

1. An apparatus for dielectrophoretic manipulation of at least one chemical species including:

a housing for containing first and second materials, said first and second materials having different dielectric constants, at least one of said first and second materials corresponding to said chemical species to be manipulated,

means for applying a non-uniform electrical field to said first and second materials for varying the relative positions of said first and second materials within said housing as a result of dielectrophoretic forces resultant from said applied non-uniform electrical field to transport said at least one chemical species to at least one predetermined location within said housing for performing a selected operation on said chemical species at said predetermined location within said housing,

whereby the position of said at least one chemical species is manipulated to said predetermined location within said housing as a result of said dielectrophoretic forces applied thereto.

2. The apparatus of claim 1 further including means for adjusting said non-uniform field applied to said first and second materials for rearranging the relative positions of said first and second materials within said housing.

3. The apparatus of claim 1 further including a plurality of materials within said housing, at least one of said materials having a dielectric constant differing from the dielectric constant of the remainder of said plurality of materials, said remainder of materials corresponding to chemical species to be manipulated within said housing.

4. The apparatus of claim 3 wherein each of said plurality of materials within said housing has a dielectric constant different from the dielectric constant of each of the other materials within the housing.

5. The apparatus of claim 1 wherein said housing includes an analyzer for analyzing said at least one chemical species, said analyzer being positioned in said predetermined location within said housing, whereby said chemical species may be manipulated into said analyzer for analysis thereof.

6. The apparatus of claim 1 wherein said housing includes means for inducing a chemical reaction in said chemical species in said predetermined location within said housing, whereby said dielectrophoretic forces are used to manipulate said chemical species into said predetermined location for inducing a chemical reaction.

7. The apparatus of claim 3 wherein said housing includes means for inducing a chemical reaction between at least two of said materials corresponding to chemical species in said predetermined location within said housing, whereby said dielectrophoretic forces are used to manipulate said at least two materials into said predetermined location for inducing a chemical reaction.

8. The apparatus of claim 1 further including a discharge chamber in communication with said housing, whereby said dielectrophoretic forces resultant from said applied non-uniform electrical field are used to manipulate said chemical species from said housing to said discharge chamber.

9. The apparatus of claim 1 further including an inlet chamber in communication with said housing, whereby dielectrophoretic forces resultant from said applied non-uniform electrical field are used to manipulate said chemical species from said inlet chamber into said housing.

10. The apparatus of claim 6 wherein said means for inducing said chemical reaction includes means for varying the temperature of said chemical species.

11. The apparatus of claim 1 wherein said housing includes means for chemical synthesizing located in said predetermined location, whereby said chemical species in said housing can be manipulated into said predetermined location for performing chemical synthesis.

12. The apparatus of claim 1 further including a plurality of materials corresponding to chemical species to be manipulated, said housing including a mixing chamber defined at said predetermined location therein, whereby said plurality of chemical species can be manipulated into said mixing chamber by said dielectrophoretic forces for mixing thereof.

13. The apparatus of claim 1 wherein said means for applying said non-uniform electrical field includes at least a first pair of opposed electrodes located at a first position within said housing, at least a second pair of opposed electrodes located at a second position within said housing, and a gate electrode disposed between said first and second pairs of opposed electrodes.

14. The apparatus of claim 13 including means for selectively adjusting the charge on said first and second pairs of opposed electrodes and on said gate electrode for controlling the flow of one of said first and second materials though said housing.

15. The apparatus of claim 13 including means for selectively adjusting the charge on said first and second pairs of opposed electrodes and on said gate electrode to separate a portion of one of said first and second materials from the remainder of such material.

16. A method of manipulating at least one chemical species comprising the steps of:

providing first and second materials within a housing, said first and second materials having different dielectric constants, one of said first and second materials corresponding to said at least one chemical species to be manipulated within said housing,

applying a non-uniform electrical field to said first and second materials to vary the relative position of said first and second materials within said housing as a result of dielectrophoretic forces resulting from said applied non-uniform electrical field to thereby vary the position of said at least one chemical species within said housing,

transporting said at least one chemical species by said dielectrophoretic forces acting thereon to at least one predetermined position within said housing, and

performing a predetermined operation on said at least one chemical species at said predetermined location within said housing.

17. The method of claim 16 further including the step of varying said applied non-uniform electrical field to vary the relative positions of said first and second materials within said housing.

18. The method of claim 16 including the step of analyzing said chemical species at said predetermined location within said housing.

19. The method of claim 16 further including the step of inducing a chemical reaction in said chemical species at said predetermined location within said housing.

20. The method of claim 16 further including the step of mixing at least two chemical species at said predetermined location within said housing.