Method and device for detecting glucose concentration

• US 4,390,621 A
• Filed: 03/01/1982
• Issued: 06/28/1983
• Est. Priority Date: 12/15/1980
• Status: Expired due to Fees

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Bauer, Robert
• Primary Examiner(s): Schor, Kenneth M.
• Application Number:
  US06/353,748
• Time in Patent Office: 484 Days
• Field of Search: 436/95, 435/14, 435/25, 435/28, 435/805, 435/810, 422/56, 422/57, 427/2, 427/3
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A method and test device for determining the glucose concentration in a test sample containing from 1/2 to 10 percent glucose are disclosed. The method and test device involve impregnating the carrier with an enzymatic testing composition which includes glucose oxidase, peroxidase and a chromogen. The carrier is subsequently impregnated with from 0.5 to 1.5 percent by weight polystyrene. Contact of the carrier with a glucose-containing test sample produces a detectable response whereby the glucose concentration can be determined.

Claims

1. In a method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which method includes the steps of;

contacting said sample with a test device which includes a carrier matrix impregnated with an enzymatic testing composition including glucose oxidase, peroxidase, and a chromogen; and

observing a detectable response produced by said test device;

the improvement which comprises impregnating the carrier matrix with a solution of from about 0.5 percent to 1.5 percent by weight of polystyrene subsequent to the impregnating of the carrier matrix with said testing composition.

2. A method as claimed in claim 1 wherein the carrier is paper.

3. A method as claimed in claim 1 wherein polystyrene is present in an amount of about 1.0 percent by weight.

4. A method as claimed in claim 1 wherein the chromogen is a benzidine-type indicator or a substituted aniline indicator.

5. A method for determining the glucose concentration in a test sample containing from about 1/2 to 10 percent glucose which comprises the steps of providing a test device which includes a carrier having inpregnated therein an enzymatic testing composition comprising glucose oxidase, peroxidase, and a chromogen;

subsequently impregnating the carrier with from 0.5 percent to 1.5 percent by weight of a solution of polystyrene;

contacting said test sample with the carrier of said test device; and

observing a detectable response, whereby the glucose concentration is determined.

6. A method as claimed in claim 5 wherein the carrier is paper.

7. A method as claimed in claim 5 wherein the polystyrene is present in an amount of about 1.0 percent by weight.