Neisseria bacteria species identification and beta lactamase testing methods
First Claim
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1. A method for providing rapid, high intensity, visual indication of the presence of Neisseria bacteria species in a specimen comprising:
- the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with at least one carbohydrate substrate taken from the class consisting of glucose, maltose, sucrose and lactose, permitting the reagent system specimen to react with the contacted carbohydrate substrate, and visually observing the reaction results for color change in a predetermined time period.
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Abstract
Method for rapid, high intensity visual indication of the presence of Neisseria bacteria species utilizing a new and improved peptone buffer and pH indicator reagent system as the specimen carrier for oxidation series testing, which series test may also include beta lactamase enzyme detection utilizing the reaction of the same reagent system carrying the specimen with a penicillin substrate.
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Citations
35 Claims
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1. A method for providing rapid, high intensity, visual indication of the presence of Neisseria bacteria species in a specimen comprising:
- the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with at least one carbohydrate substrate taken from the class consisting of glucose, maltose, sucrose and lactose, permitting the reagent system specimen to react with the contacted carbohydrate substrate, and visually observing the reaction results for color change in a predetermined time period.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 13. The method of rapid, high intensity, visual indication of the presence of beta lactamase enzymes in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, contacting the reagent system specimen suspension with a penicillin substrate, permitting the reagent system specimen to react with the contact penicillin substrate, and visually observing the reaction results for color change in a predetermined time period.
- 24. A method of rapid, high intensity, visual indication of the presence of Neisseria bacteria species and of beta lactamase enzyme in a specimen comprising the steps of admixing the specimen with a peptone buffer and pH indicator reagent system to form a specimen suspension, which is contacted with a penicillin substrate, and with carbohydrate substrates separately, permitting the reagent system specimen to react with the contacted carbohydrate substrates and the penicillin substrate, and visually observing the reaction results for color change induced by the presence of one or more enzymes taken from the class consisting of N. gonorrhoeae, N. meningitidis, N. lactamica, and N. sicca, in a predetermined time period.
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25. The method of claim 25 wherein a portion of the reagent system specimen suspension is contacted with each of said carbohydrate substrates for reaction of the reagent system specimen with the contacted carbohydrate substrate for visual observation of the reaction results for color change.
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