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Cofactor indicator compositions

  • US 4,394,444 A
  • Filed: 06/21/1982
  • Issued: 07/19/1983
  • Est. Priority Date: 06/21/1982
  • Status: Expired due to Fees
First Claim
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1. A composition for the determination of an analyte in a sample which composition comprises:

  • an analyte-responsive component comprising a pyridine nucleotide susceptible of reduction in response to the presence of said analyte and at least one constituent interreactive with said analyte to cause reduction of the pyridine nucleotide;

    a hydroxylase capable of being uncoupled and a pseudosubstrate capable of uncoupling said hydroxylase, which hydroxylase and pseudosubstrate, in the presence of the reduced form of said pyridine nucleotide, are effective to generate hydrogen peroxide;

    a peroxidatively active substance; and

    a hydrazone indicator comprising a hydrazone and a coupler, which indicator, when in its oxidized form, cannot be reduced by said pyridine nucleotide.

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