Culture medium and conditions for growth of magnetic bacteria
First Claim
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1. An aqueous culture medium for the growth of a biologically pure culture of magnetic bacteria, comprising, per 100 ml, about 2-30 μ
- M of ferric quinate, about 10-1000 mg of an organic compound selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
-hydroxybutyric acid, maleic acid, galactose, rhamnose, melibiose, acetic acid, adipic acid, and glutaric acid, a vitamin source, a mineral source, a nitrogen source, an acetate source, and a pH buffer, said pH buffer resulting in a pH of said aqueous culture medium of about 5.2-7.5.
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Abstract
A biologically pure culture of a bacterium of the genus Aquaspirillum, designated MS-1, has been found to contain chains (so-called magnetosomes) of single domain magnetite particles. The magnetite particles are roughly cubic and are about 500 A on a side. Each of the chains contains approximately 20 magnetite particles. These magnetite particles can be recovered from the bacterium and usefully employed in magnetic recording devices and the like. The biologically pure culture of the magnetic bacterium may be grown in a low oxygen environment and on a nutrient medium containing ferric quinate.
11 Citations
27 Claims
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1. An aqueous culture medium for the growth of a biologically pure culture of magnetic bacteria, comprising, per 100 ml, about 2-30 μ
- M of ferric quinate, about 10-1000 mg of an organic compound selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
-hydroxybutyric acid, maleic acid, galactose, rhamnose, melibiose, acetic acid, adipic acid, and glutaric acid, a vitamin source, a mineral source, a nitrogen source, an acetate source, and a pH buffer, said pH buffer resulting in a pH of said aqueous culture medium of about 5.2-7.5. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 24, 25, 26, 27)
- M of ferric quinate, about 10-1000 mg of an organic compound selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
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9. An aqueous culture medium for the growth of a biologically pure culture of magnetic bacteria, comprising, per 100 ml, about 2-30 μ
- M of ferric quinate, about 10-1000 mg of an organic acid selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
-hydroxybutyric acid, and maleic acid, a vitamin source, a mineral source, about 1-100 mg of an ammonium ion or nitrate ion source, about 2-100 mg of acetic acid or a salt of acetic acid, about 0.1-5 mM of a phosphate buffer, about 2.5-50 mg of sodium thioglycolate, and the remainder substantially distilled water. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
- M of ferric quinate, about 10-1000 mg of an organic acid selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
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17. An aqueous culture medium for the growth of a biologically pure culture of magnetic bacteria, comprising, per 100 ml, about 25 μ
- M ferric quinate, about 100 mg of an organic acid selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
-hydroxybutyric acid, and maleic acid, a vitamin source, a mineral source, about 10 mg of a nitrate source, about 20 mg of acetic acid or a salt of acetic acid, about 5 mM of a phosphate buffer, about 5 mg of sodium thioglycolate, and the remainder substantially distilled water. - View Dependent Claims (18, 19, 20, 21, 22, 23)
- M ferric quinate, about 100 mg of an organic acid selected from the group consisting of fumaric acid, tartaric acid, malic acid, succinic acid, lactic acid, pyruvic acid, oxaloacetic acid, malonic acid, β
Specification