Focused aperture module

• US 4,395,676 A
• Filed: 11/24/1980
• Issued: 07/26/1983
• Est. Priority Date: 11/24/1980
• Status: Expired due to Term

• Current Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Original Assignee: Coulter Electronics, Inc. (Danaher Corporation)
• Inventors: Hollinger, John D., Groves, Michael R., Hogg, Walter R.
• Primary Examiner(s): Tokar, Michael J.
• Application Number:
  US06/209,611
• Time in Patent Office: 974 Days
• Field of Search: 324/71 CP, 235/92 PC
• US Class Current: 324/71.4
• CPC Class Codes: G01N 15/13 Details pertaining to apertures
• CPC Class Codes: G01N 2015/1019 Associating Coulter-counter and optical flow cytometer [OFC]

Abstract

A particle analyzer for a sample suspension of particles originating in a container in which: a flow director is mounted between the container and a first chamber, a particle sensing aperture is mounted between the first chamber and a second chamber, a liquid sheath is introduced into the first chamber for hydrodynamically focusing the particles through the sensing aperture, an electrical current passes through the sensing aperture for generating impedance signals, the pressure drop is regulated across the flow director to assure a constant flow of the suspension through the flow director and the aperture, and the flow director is formed into an optical element for viewing the sensing aperture.

Claims

1. A particle analyzing apparatus for detecting properties of particles, said particle analyzing apparatus including a container for holding a liquid suspension of the particles to be analyzed, a first liquid holding chamber, a flow director having a bore which forms a liquid passageway between said container and said first chamber, a second liquid holding chamber, aperture means including a sensing aperture which forms a constricted liquid and electrical passageway between said first chamber and said second chamber, means for providing an electrical current through said sensing aperture to generate detectable electrical signals with the passage of the particles through said sensing aperture, fluid moving means for providing a pressure drop across said sensing aperture to move a quantity of liquid from said first chamber to said second chamber, means for providing a particle free liquid to said first chamber to form a liquid sheath around a quantity of said liquid suspension from said flow director, the improvement comprising:

means for providing a substantially constant pressure drop across said flow director to move said quantity of said liquid suspension from said container to said first chamber, whereby said substantially constant pressure drop establishes a substantially constant flow rate of said liquid suspension through said sensing aperture;

said bore of said flow director having an elongated configuration dimensioned to form a fluid resistor for limiting the flow of said quantity of said suspension through said bore, whereby said fluid resistor sufficiently restrains the flow therethrough to a desired flow rate so as to allow for said pressure drop across said flow director to be of a magnitude that is subject to regulation and measurement.

2. The particle analyzing apparatus according to claim 1, wherein said means for providing a pressure drop across said flow director is operative to provide a pressure drop of at least 1 inch of mercury.

3. The particle analyzing apparatus according to claim 1, wherein said means for providing a particle free liquid to said first chamber includes a liquid reservoir in fluid communication with said first chamber;

said means for providing a pressure drop across said flow director includes means for maintaining said liquid reservoir at a predetermined pressure; and

said apparatus further includes means for maintaining said container at a predetermined pressure which is higher than said pressure of said liquid reservoir.

4. The particle analyzing apparatus according to claim 3, wherein said means for maintaining said container at said predetermined pressure comprises said container being exposed to the atmosphere.

5. The particle analyzing apparatus according to claim 1, further comprising,

a manometer operative to measure the pressure drop acorss said flow director.

6. The particle analyzing apparatus according to claim 5, further comprising,

said manometer having one end in communication with said first chamber and the other end opened to the atmosphere.

7. The particle analyzing apparatus according to claim 1 or 5, wherein a director holder defines a wall between said container and said first chamber and has said flow director mounted therein;

said means for providing an electrical current through said sensing aperture includes a first electrode disposed in said container, a second electrode disposed in said second chamber, and a third electrode mounted in said director holder; and

said third electrode has a portion in fluid communication with said container and a portion in fluid communication with said first chamber, whereby said third electrode electrically shunts said liquid passageway formed by said flow director.

8. The particle analyzing apparatus according to claim 7, wherein said third electrode has an electrical resistance substantially less than the electrical resistance of said liquid passageway formed by said director bore.

9. The particle analyzing apparatus according to claim 7, wherein said portion of said third electrode in fluid communication with said container comprises a relatively thin first electrode portion mounted over a majority portion of the wall of said director holder facing said container, said portion of said third electrode in fluid communication with said first chamber comprises a relatively thin second electrode portion mounted over a majority portion of the wall of said director holder facing said first chamber, and said third electrode includes an interconnecting electrode portion which passes through said director holder and electrically couples said first electrode portion with said second electrode portion.

10. The particle analyzing apparatus according to claim 1 or 5 wherein said director holder is removably mounted between said container and said first chamber.

11. The particle analyzing apparatus according to claim 10, wherein said director holder has a disk-like configuration with a circular periphery, said periphery has threads formed thereon, and a juncture region between said container and said first chamber has threads formed therein for mating in rotatable engagement with said threads of said director holder.

12. The particle analyzing apparatus according to claim 1 or 5, wherein said flow director is formed of an optically transparent material, said flow director has a pair of optical lens surfaces, said optical lens surfaces of said flow director and said sensing aperture are coaxially aligned on an optical axis, and further including,

a source of light aligned to provide an illuminating beam along said optical axis in the direction of said flow director and said sensing aperture, and

an optical detection assembly positioned on the other side of said flow director and said sensing aperture relative to the side having said source of light,

whereby monitoring of said sensing aperture can be accomplished.

13. The particle analyzing apparatus according to claim 12, wherein each said optical lens surface has a planar configuration.

14. For use in a particle analyzing apparatus of the Coulter type in which a particle sensing aperture provides the only fluid and electrical current paths between a body of liquid in a first chamber and a body of liquid in a second chamber, a sample suspension being caused to flow through said sensing aperture and into said second chamber;

said apparatus also including a bore opening from a container into said first chamber for receiving the flow of sample suspension, particle free liquid being supplied into said first chamber for sheathing said flowing sample suspension as it passes from said bore;

the improvement comprising, means for providing a relatively constant pressure drop across said bore to move said sample suspension from said container to said first chamber, whereby said substantially constant pressure drop establishes a substantially constant flow rate of said sample suspension through said sensing aperture;

said bore being dimensioned to form a fluid resistor for limiting the flow of said sample suspension through said bore.