Luminescence detection device

• US 4,396,579 A
• Filed: 08/06/1981
• Issued: 08/02/1983
• Est. Priority Date: 08/06/1981
• Status: Expired due to Fees

• Current Assignee: Miles Laboratories, Inc. (Bayer AG)
• Original Assignee: Miles Laboratories, Inc. (Bayer AG)
• Inventors: Vogelhut, Paul O., Schroeder, Hartmut R.
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US06/290,656
• Time in Patent Office: 726 Days
• Field of Search: 422/52, 422/61, 422/68, 422/102, 422/104, 422/66, 23/230 R, 23/230 B, 435/8, 435/35, 435/291, 436/135, 436/172, 436/546
• US Class Current: 422/52
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/835 Bacillus circulans
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide

Abstract

A luminescence detection device for quantitatively detecting an analyte in a liquid sample, which device comprises (1) a reaction compartment having, along a primary axis, opposite end portions, a first of which is for introduction of fluid reagents and sample into the compartment and the other of which forms a light transmissive aperture of predetermined size, the compartment being suitable to hold a composition which luminesces in response to contact with analyte-containing sample; (2) closure means in said first end portion for admitting a cannula, whereby fluid is introduced into said compartment, and for closing the reaction compartment; (3) a photoresponsive imaging layer; (4) means for associating the photoresponsive imaging layer and the reaction compartment such that the photoresponsive imaging layer is positioned preferably substantially perpendicular to the primary axis of the reaction compartment, at a predetermined distance from the end portion forming the aperture so as to be exposed to light emanating therefrom; and (5) means for preventing exposure of the photoresponsive imaging layer to ambient light.

Claims

1. A luminescence detection device for quantiatively detecting an analyte in a liquid sample, which device comprises

(1) an opaque reaction compartment having, along a primary axis, opposite end portions, a first of which is for introduction of fluid reagents and sample into the compartment and the other of which forms a light transmission aperture of predetermined size, the compartment being suitable to hold a composition which luminesces in response to contact with analyte-containing sample;

(2) closure means in said first end portion for admitting a cannula, whereby fluid is introduced into said compartment, and for closing the reaction compartment;

(3) a photoresponsive imaging layer;

(4) means for associating the photoresponsive imaging layer and the reaction compartment such that the photoresponsive imaging layer is positioned at a predetermined distance from the end portion forming the aperture so as to be exposed to light emanating therefrom; and

(5) means for blocking the photoresponsive imaging layer from exposure to ambient light.

2. The device of claim 1 wherein the reaction compartment comprises an opaque reaction vessel housing and a reaction vessel therein.

3. The device of claim 2 wherein the reaction vessel is transparent.

4. The device of claim 1 wherein the closure means is an integral portion of the compartment.

5. The device of claim 1 wherein the closure means is a septum.

6. The device of claim 1 wherein the photoresponsive imaging layer is photographic film.

7. The device of claim 1 wherein the photoresponsive imaging layer is self-developing photographic film.

8. The device of claim 1 wherein the means for associating the photoresponsive imaging layer and reaction compartment comprises a base having a horizontal chamber for accepting the photoresponsive imaging layer and, thereabove, a carriage for vertically holding the reaction compartment.

9. The device of claim 8 wherein the base further comprises a shutter assembly positioned between the reaction compartment and the photoresponsive imaging layer.

10. The device of claim 8 wherein the base blocks the photoresponsive imaging layer from exposure to ambient light.