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High molecular polysaccharide MPS-80

  • US 4,396,763 A
  • Filed: 11/17/1981
  • Issued: 08/02/1983
  • Est. Priority Date: 01/14/1981
  • Status: Expired due to Fees
First Claim
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1. High molecular polysaccharide MPS-80 having the following physicochemical properties:

  • (1) elementary analysis;

    C;

    42.2%H;

    6.9%O;

    50.4%(2) molecular weight;

    (i) molecular weight determined by ultrafiltration method by means of Sepharose 2B as shown in FIG. 1;

    said polysaccharide is fractionated near the void volume under conditions comprising 2.5×

    40.5 cm column size, 5 g fraction, 2.5 mg (1 ml) sample loaded and 0.05 M phosphate buffer solutin (pH 6.0) used as developer;

    (ii) molecular weight determined by ultracentrifugation whereina sample is dissolved in 0.2 M phosphate buffer solution (pH 7.3) and the resulting solution having a concentration of 0.1% is subjected to the ultracentrifugation (preset rotation rate;

    51,200 RPM) to obtain a sedimentation constant of 7.98S (S;

    Svedberg unit), the sedimentation pattern being single;

    (3) melting point (decomposition point);

    said polysaccharide begins to discolor at around 262°

    C. and is blackened at 263°

    -264°

    C.(4) specific rotatory power;

    
    
    space="preserve" listing-type="equation">[α

    ].sub.D.sup.18 =+33.2 (C═

    0.5%)(5) ultraviolet ray absorption spectrum;

    as shown in FIG. 2.(6) infrared ray absorption spectrum;

    as shown in FIG. 3.(7) solubilities in solvents;

    soluble in water but insoluble in methanol, ethanol, acetone and ether;

    (8) color reactions;

    (i) Molisch reaction;

    +(ii) Anthrone reaction;

    +(iii) Cysteine sulfuric acid reaction;

    +(iv) Aniline hydrochloric acid reaction;

    -(v) Carbazole sulfuric acid reaction;

    -(vi) Elson-Morgan reaction;

    -(vii) Biuret reaction;

    -(9) basicity, acidity or neutrality;

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